DNA repair capacity of eukaryotic cells has been studied extensively in recent years. Mammalian cells have been engineered to overexpress recombinant nuclear DNA repair proteins from ectopic genes to assess the impact of increased DNA repair capacity on genome stability. This approach has been used in this study to specifically target O-methylguanine DNA methyltransferase (MGMT) to the mitochon...

The Ventilator Manager (VM) program is an experiment in expert system development that builds on our experience with rules in the MYCIN system. VM is designed to interpret on-line quantitative data in the intensive care unit (ICU) of a hospital. After a major cardiovascular operation, patient often needs mechanical assistance with breathing and is put in the ICU so that many parameters can be m...

Tafazzin, a mitochondrial acyltransferase, plays an important role in cardiolipin side chain remodeling. Previous studies have shown that dysfunction of tafazzin reduces cardiolipin content, impairs mitochondrial function, and causes dilated cardiomyopathy in Barth syndrome. Reactive oxygen species (ROS) have been implicated in the development of cardiomyopathy and are also the obligated byprod...

The use of artificial intelligence intelligence (AI) in medicine can be traced back to 1968 when Paycha published his paper Le diagnostic a l’aide d’intelligences artificielles: presentation de la premiere machine diagnostri [1]. Few years later Shortliffe et al. [2] presented an expert system named Mycin which was able to identify bacteria causing severe blood infections and to recommend antib...

This paper presents an improving knowledge base and inference engine of a medical expert system for diagnosing depressive disorders. calls PORUL.DEP. PORUL.DEP’s includes more than 850 positive rules. PORUL.DEP has been tested on 260 records depressed patients. gives correct diagnosis 95% with light disorder without disorder, but the remaining disorders are not accurate. Average percent 24 %. A...

Resistance to the cytotoxic effects of daunomycin and Adria mycin (ADR) in sublines of Ehrlich ascites and P388 mouse tumors has been demonstrated to be due to reduced cellular accumulation and retention of drug. In this study, the effect of the calmodulin inhibitor trifluoperazine on the cellular accumula tion, retention, and cytotoxic effects of ADR in ADR-sensitive (P388/S) and ADR-resistant...

Since graduate school at the University of Missouri, Cole Haynes has been preoccupied with how mixed up, misfolded proteins inside organelles affect the well-being of cells. At Missouri, he studied the classic pathway: how rumpled proteins are recognized in the yeast endoplasmic reticulum and ultimately targeted for degradation. As a postdoctoral fellow, he went a little more cosmopolitan, movi...

I t has been reported that excessive intake of sugar-containing soft drinks can result in diabetic ketoacidosis or ketosis in obese patients with type 2 diabetes (1). We describe herein soft drink ketosis–onset diabetes in a nonobese patient with mito-chondrial myopathy, lactic acidosis, and stroke-like episodes (MELAS). A 27-year-old Japanese man consulted a local otolaryngeal clinic with a hi...

This paper examines the quantities used by MYCIN to reason with uncertainty, called certainty factors. It is shown that the original definition of certainty factors is inconsistent with the functions used in MYCIN to combine the quantities. This inconsistency is used to argue for a redefinition of certainty factors in terms of the intuitively appealing desiderata associated with the combining f...