نتایج جستجو برای: pcr analysis to extract total rna
تعداد نتایج: 11639216 فیلتر نتایج به سال:
conclusions the analysis indicates that the hev isolated from blood donors in the southwest of iran belongs to genotype 1. however, more samples from other geographic regions of iran are needed to confirm these findings. because transmission of hev by administration of blood or blood components is likely to occur, it may be sensible to screen donor blood for hev to eliminate transfusion-transmi...
abstract this study evaluates the iranian pre-university english textbook in terms of needs, objectives, content, and methodology. the study was designed on the qualitative- quantitative survey basis using interviews and questionnaires, a researcher-made textbook evaluation checklist and a needs analysis questionnaire. the textbook evaluation questionnaire was used in this study to elicit the ...
During 2010-2011, Real-time PCR procedure was used to detecting FMDV RNA on 147 epithelium samples from the field. In this survey, for Real-time PCR from 3D gene segment as conserve region selected for tracking all of seven serotypes FMDV. The assay detected the viral RNA in all serotypes of FMDV. The rRT-PCR specifically detected FMD virus in sample with greater sensitivity than our convention...
با توجه به این که استفاده بیش از حد از مواد شیمیایی در کشاورزی جهت کنترل بیماری ها سبب آلودگی محیط زیست شده و همچنین پاتوژن ها به سرعت به این مواد شیمیایی مقاومت حاصل می کنند، پیدا کردن روشی جایگزین جهت مقابله با بیماریها امری ضروری به نظر می رسد. ژن های مقاومت گیاهان (r gene)، منابع ژنتیکی با ارزشی هستند که میتوان از آن ها در این راستا استفاده کرد. این تحقیق با هدف آنالیز مولکولی چند رقم برنج ...
Background: To evaluate the radioprotective potential of Punica granatum fruit rind extract (PGRE) in mouse testes. Materials and Methods: Adult male mice were divided into four groups. Group I was kept without any treatment. Group II was irradiated with 8Gy Co60 gamma irradiation and Group III was given PGRE one hour before irradiation. Group IV was fed with PGRE at the rate of 10 mg/kg body w...
Fusarium head blight (FHB) caused by Fusarium graminearum is responsible for billions of dollars in agriculture losses. The goal of the present study was evaluation the expression of acidic chitinase, one of PR proteins, in wheat defense response against different FHB induced treatments in 'Falat' as a highly susceptible and 'Sumai3' as a tolerant cultivar. These treatments contained fungi extr...
Viral myocarditis is a moderate disease, but it sometimes causes progressive cardiac disorder. Many different viruses have been considered as the agent of viral myocarditis, but Coxsackievirus of the B group, in particular of the Coxsackievirus B3 (CVB3), is more than fifty percent of cases of viral myocarditis. CVB3 is a positive single-stranded RNA virus and a member of the genus Enterovirus ...
Objective: This work aimed to enhance colony polymerase chain reaction (PCR) for the 16S rRNA of several Escherichia coli strains. Methods: The isolation E. is done from gut chicken and soil. Then, we optimized condition PCR amplification 16s ribosomal RNA. We successfully designed primer 3 RNA made dilution solution with grade water that 1:10. Moreover, finally, a 20 μL contains master mix our...
this study was intended to analyze the listening tapescripts of the elementary and pre-intermediate levels of total english textbooks from the pragmatic dimension of language functions and speech acts in order to see whether the listening tasks are pragmatically informative or not. for this purpose, 8 conversations from the two books were selected randomly, and then, the two pragmatic models of...
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