Objective(s): To reveal the detailed mechanism underlying the functions of salidroside on the inflammation of intestinal epithelial cells during IBD.Materials and Methods: Quantitative real-time PCR was employed to assess the expression of IL-6, IL-10, and α-defensins 5 and 6. ELISA assay was performed to measure the secretion of IL-6 and IL-10. MTT assay was used to determine the cell viabilit...

Abstract Lysine-ε-acetylation (Kac) is a post-translational modification (PTM) that critical for metabolic regulation and cell signaling in mammals. However, its prevalence importance plants remain to be determined. Employing high-resolution tandem mass spectrometry, we analyzed protein lysine acetylation five representative Arabidopsis organs with 2 ~ 3 biological replicates per organ. A total...

Abstract One of the most severe side effects systemic lupus erythematosus (SLE) is nephritis (LN). To search for potential therapeutic targets in SLE crucial progression SLE. In this study, we selected C57BL/6J mice as controls and MRL/lpr an LN model obtained dual specificity phosphatase 2 (DUSP2)-overexpressed by injecting AAV-DUSP2 plasmid into tail vein. Then, proteinuria, urea nitrogen, ds...

Plants to survive against devastating impact of invasive biotic agents have powerfully struggle in armed combat with microorganisms. Therefore they need activate rapidly and efficiently pre-existing potential defensive chemicals. After upon perception initial external stimuli through cell membrane receptors and/or cytoplasmic resistance proteins before activity related genes, some participated ...

Non-muscle myosin 2A (NM2A) is a key cytoskeletal enzyme that, along with actin, assembles into actomyosin filaments inside cells. NM2A fundamental for cell adhesion and motility, playing important functions in different stages of development during the progression viral bacterial infections. Phosphorylation events regulate activity cellular localization NM2A. We previously identified tyrosine ...

This article mainly discusses the mechanism of SINOMENINE nanoparticles in treatment breast cancer by using network pharmacology method. TCMSP and Swiss Target Prediction databases were searched for target SINOMENINE. The was through GeneCards database. common targets both considered as treating cancer. software Cytoscape 3.7.1 used topological analysis visualization. DAVID GO enrichment analys...

Objective(s): To understand the role of mitochondrial respiration in cisplatin sensitivity, we have employed wild-type and mitochondrial DNA depleted Rho0 yeast cells. Materials and Methods: Wild type and Rho0 yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against cisplatin by monitoring growth curves, oxygen consumption, pH changes ...

protein tyrosine phosphatases (ptpases) regulate the tyrosine phosphorylation of target proteins in volved in several biological activities including cell proliferation and transformation. protein tyrosine phosphatase e (ptpe) contains duplicated ptpase-like domains and a short extracellular region. us ing the fluorescence in situ hybridization method, the gene encoding ptpe (locus symbol ptpre...

Abstract Protein homeostasis is modulated by stress response pathways and its deficiency a hallmark of aging. The integrated (ISR) conserved stress-signaling pathway that tunes mRNA translation via phosphorylation the initiation factor eIF2. ISR activation are finely balanced eIF2 kinases guanine nucleotide exchange eIF2B. However, role during aging remains poorly understood. Using genomic muta...