Abstract In the course of identifying molecular mechanism that is related to strong cell‐cell adhesion in stratified structures squamous epithelium, calmodulin‐like protein 5 (CALML5) was identified as a spinous structure‐associated by producing monoclonal antibodies with use crude intercellular portion tissue an immunogen and subsequent morphologic screening. By electrophoretic mobility shift ...

the aim of this study was to analyze the polymorphisms existing in the 5´ flanking region, exonic, and some parts of the intronic regions as well as methylation analysis of the ednrb gene in cattle and goat. these regions were sequenced in three different breeds of cattle including sistani, golpayegani, and holstein and were compared with that of marghoz goat. the results identified that this g...

Introduction Genetic background has known to be associated with the outcome of human T cell lymphotropic virus (HTLV) type I infection. In The present study we investigate the association between GM-CSF gene polymorphisms with the outcome of HTLV-I infection. Materials and Methods We analyzed 3 single-nucleotide polymorphisms in the promter region of granulocyte macrophage colony stimulating...

Background: Promoter hyper-methylation of tumor suppressor genes is a common event that occurs in cancer. As methylation is a reversible modification, agents capable of reversing an abnormal methylation status should help to combat cancer. 5-Aza-CdR is a DNA methyl-transferase inhibitor. The present study aimed to evaluate the effect of 5-Aza-CdR on the proliferation of human pancreatic cancer ...

We describe a new biochemical technique, "promoter competition assay," for examining the role of cis-acting DNA elements in tissue cultures. Recent advances in tissue engineering permit the culture of a variety of cells. Many tissues are engineered, however, without an appropriate understanding of molecular machinery that regulates gene expression and cellular growth. For elucidating the role o...

In order to develop a rapid and versatile assay system suitable for the analysis of regulated expression of tobacco pathogenesis-related protein 1a (PR-1a) gene, we investigated the use of the transient gene expression system in tobacco BY-2 cells by microprojectile bombardment. Using dual luciferase assay as a reporter gene expression detection system, we observed significant induction of PR-1...

objective(s): phospholipase c ζ (plcζ) is considered as a nominee for sperm associated oocyte activating factors and is located back-to-back with capza3, an actin-capping protein controlling actin polymerization during spermiogenesis. they contain a common bidirectional promoter. the objective of this study was to identify individuals with parallel low expression of plcζ and capza3 mrna, in hop...

Abstract The Fusobacterium genus contains Gram-negative, rod-shaped anaerobic bacteria. necrophorum and its two subspecies, funduliforme, are normal ruminal inhabitants of cattle. Subsp. is the primary causative agent liver abscesses in feedlot varium also a rumen inhabitant, but role fermentation involvement unknown. Currently, no PCR assays exist to quantify F. both subspecies necrophorum. Th...

Melatonin is widely detected in nature. It has pleiotropic activities, mediated by interactions with high affinity G-protein coupled MT1 and MT2 receptors, or which at concentration (≥1 μM) are independent. The AhR a perfect candidate for receptor mediating latter effects because melatonin structural similarity to its natural ligands. Our molecular modeling, using homology crystal structure of ...

Claudin-5 is an integral membrane protein and a critical component of endothelial tight junctions that control paracellular permeability. Claudin-5 is expressed at high levels in the brain vascular endothelium. Estrogens have multiple effects on vascular physiology and function. The biological actions of estrogens are mediated by two different estrogen receptor (ER) subtypes, ER alpha and ER be...