Water and air pollutants have huge impacts on the entire living system. In addition, the newly emerging nanopollutants, increasing global warming, and consequent climate changes are posing major threats to the freshwater or fresh air availability. This has made it urgent to invent an appropriate water/air treatment technology that removes nanopollutants and also desalinates water to a significa...

Affinity chromatography is a method which depends essentially on the interaction between the molecule to be purified and a solid phase that will allow the separation of contaminants. Lectins are carbohydrate-binding proteins which can be purified by affinity chromatography; also, the presence of multiple molecular forms of lectins in a preparation can be separated. Immobilized lectins have been...

The amiloride-binding Na+ channel protein of high electrical resistance epithelia was solubilized and purified from cultured A6 toad kidney cells and bovine renal papilla. Purification was assessed by enrichment in [3H]methylbromoamiloride specific binding. Chromatography of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)-solubilized plasma membrane vesicles on agarose-immobil...

Bacteria are the predominant source for producing recombinant proteins but while many exogenous proteins are expressed, only a fraction of those are soluble. We have found that a new actin regulatory enzyme Mical is poorly soluble when expressed in bacteria but the use of a Nus fusion protein tag greatly increases its solubility. However, available vectors containing a Nus tag have been enginee...

Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion protein with His-tag for simple production and rapid purification. Materials and Methods: After PCR a...

background and objective: neisseria meningitidis is a leading cause of meningitis and sepsis worldwide. the factor h binding protein (fhbp) is a key virulence factor of neisseria meningitidis that is able to selectively bind to human factor h, the key regulator of the alternative complement pathway, which it has important implications for meningococcal pathogene- sis and vaccine design. the aim...

objective: angiogenesis a process that results in neo-vascularization is an essential stage in growth of solid tumors and the formation of metastases. vascular endothelial growth factor (vegf) and its receptors, vegfr1 (flt-1) and vegfr2 (kdr/flk-1), are the major regulators for tumor angiogenesis. recent studies showed that second domain of vegfr-1is a key factor for vegf/vegfr-1 interaction. ...