نتایج جستجو برای: random primer et15
تعداد نتایج: 314077 فیلتر نتایج به سال:
Recogniting the genetic diversity and classification of inheritancepools is one of important activities in the field of breeding and maintaining genetic resources in plants. In order to assess the genetic diversity in 48 genotypes of soybean amplification of gene loci was performed by using 10 ISSR primers In Genetics and Plant Breeding laboratory, College of Agriculture andNatural Resources, U...
The reported genome-walking methods still suffer from some deficiencies, such as cumbersome experimental steps, short target amplicon, or deep background. Here, a simple and practical fork PCR was proposed for genome-walking. employs primer set of three random oligomers to implement walking task. In primary PCR, the low-stringency amplification cycle mediates binding places on genome, producing...
The poor information available on variation of Ascochyta blight fungus is the most important factor limiting chickpea breeding programs for resistance to blight disease. In this study, efforts were made to detect genetic variation of the pathogen in Iran. The RAPD marker was employed to evaluate 26 isolates collected from 16 provinces. Twelve random primers were used to analyze genomic DNA of t...
The poor information available on variation of Ascochyta blight fungus is the most important factor limiting chickpea breeding programs for resistance to blight disease. In this study, efforts were made to detect genetic variation of the pathogen in Iran. The RAPD marker was employed to evaluate 26 isolates collected from 16 provinces. Twelve random primers were used to analyze genomic DNA of t...
Jinten hitam ( Nigella sativa ) is one of the medicinal plants in family Ranunculaceae, native to Mediterranean region and several regions Asia. The seed widely cultivated, distributed, used pharmaceuticals, cosmetics, food industries. Due similar morphology its potential adulterant such as Corchorus spp., N. seeds are susceptible adulteration substitution markets. Molecular markers have become...
BACKGROUND The ability to accurately measure patterns of gene expression is essential in studying gene function. The reverse transcription polymerase chain reaction (RT-PCR) has become the method of choice for the detection and measurement of RNA expression patterns in both cells and small quantities of tissue. Our previous results show that there is a significant production of primer-independe...
three tuberculous, twenty-one non-tuberculous mycobacterial (ntm) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of dna fragments in a polymerase chain reaction with single universal primer (up-pcr). a rather wide limit of tolerance for variations in procedure of pcr mixture preparation and thermocycling parameters was found. there...
Genetic analysis in 53 early introduced clones of rubber tree (Hevea brasiliensis) collected from different areas in Southern Thailand was performed using RAPD (Random Amplified Polymorphic DNA) and microsatellite markers. Seventeen cultivated clones (34 samples) were also included to compare DNA patterns. DNA was isolated from leaf samples using CTAB buffer. One hundred and ninety two 10-base ...
ABSTRACT This study was conducted to identify the species of Colletotrichum infecting tamarillo, mango, and passiflora in Colombia and to assess whether cross-infection between host species is occurring. Isolates of Colletotrichum spp. from tamarillo (n = 54), passiflora (n = 26), and mango (n = 15) were characterized by various molecular methods and by morphological criteria. Morphological cha...
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