to investigate the genetic diversity of 21 rapeseeds (brassica napus l.), 20 semi-random isj and ssr primers were used. primers produced a total number of 158 (81.5%) polymorphic bands (51 ssr and 107 isj bands). average numbers of polymorphic bands for isj, ssr and combined of them were 10.7, 5.1 and 7.9, respectively. pic values ranged from 0.0150 for specific primer o110d08 to 0.3420 for sem...

A very sensitive and specific method for the random amplification of whole DNA molecules and genomes ranging from 400 base pairs (bp) to 40 Megabase (Mb) is described. This simple, two step PCR (1-3) strategy utilizes tagged random primers that consist of a pool of all possible 3' sequences for binding to the target DNA and a constant 5' region for the detection of incorporated primers. As litt...

A fuzzy random variable (FRV) has been conceptualized as a vague perception of a crisp but unobservable random variable (RV) and also as a random fuzzy set. Since actuaries seem receptive to these notions of FRV, and there are sources of uncertainty that RVs can not accommodate, but FRVs can, one would expect FRVs to be a component of the actuarial arsenal, but generally this is not the case. A...

The solubilization capabilities of micellar solutions of three single surfactants, two alcohol alkoxylates B048 and B266, and the tallow alkyl ethoxylated amine ET15, and their equimolar mixed solutions toward the herbicides flurtamone (FL), metribuzin (MTZ) and mesotrione (MST) were investigated. The solubilization capacity was quantified in terms of the molar solubilization ratio (MSR), criti...

Standard systematic evolution of ligands by exponential enrichment (SELEX) protocols require libraries that contain two primers, one on each side of a central random domain, which allow amplification of target-bound sequences via PCR or RT-PCR. However, these primer sequences cause nonspecific binding by their nature (generally adding about 20 nt on each end of the random sequence of about 30-4...

Beetles belong to the order Coleoptera, and are important organisms for the ecosystems functioning in which they inhabit. Taxonomists classify these organisms to facilitate their identification and study. For this purpose, they use identification keys, composed by dilemmas, which increase in number when classification approaches to the least inclusive category. This work presents a method to au...

A method has been developed to prepare random DNA fragments using PCR. First, two cycles are carried out at 16 degrees C with the Klenow's fragment and oligonucleotides (random primers) with random 3'-sequences and the 5'-constant part containing the site for cloning with the site-specific endonuclease. The random primers can link to any DNA site, and random DNA fragments are formed during DNA ...

Random Amplified Polymorphic DNA (RAPD) analysis of Pistacia vera L. was used to distinguishing sex type in progeny plants. Varied genotypes of the pistachio were analyzed by PCR amplification of a ten base primer previously found to be linked to the expression of a 945bp amplification band in female trees and its absence in males. Results revealed that genetic material from cultivar samples am...

101 isolates of brucella spp. including the reference (n=7) and the iranian field strains belonging to last years 1961-2003 (n=94) were biotyped and classified into 9 biotypes. random amplified polymorphic dna (rapd) analysis using a ten mer-ap4 primer was generated 72 reproduciable dna band. the typeability was 100%. the thirteen isolates were classified into five groups, each containing 2-4 b...

Aptamers are oligonucleotides that can bind to specific target molecules. Most aptamers are generated using random libraries in the standard systematic evolution of ligands by exponential enrichment (SELEX). Each random library contains oligonucleotides with a randomized central region and two fixed primer regions at both ends. The fixed primer regions are necessary for amplifying target-bound ...