نتایج جستجو برای: recombinant coat protein

تعداد نتایج: 1310399  

2010
Sébastien Potot Cláudia R. Serra Adriano O. Henriques Ghislain Schyns

Display of proteins like feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater efficiency in the manufacturing of animal products and minimizing the environmental 5 impact of increased animal production. To deliver their full potential in the gut, feed enzymes must survive the ha...

Journal: :iranian journal of basic medical sciences 0
roghayeh rahimi department of immunology, faculty of medical sciences, tarbiat modares university, tehran , iran massoumeh ebtekar department of immunology, faculty of medical sciences, tarbiat modares university, tehran , iran seyed mohammad moazzeni department of immunology, faculty of medical sciences, tarbiat modares university, tehran , iran ali mostafaie biotechnology research center, kermanshah university, kermanshah, iran mehdi mahdavi department of virology, pasteur institute of iran, tehran, iran

objective(s):multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (dcs) are promising approaches for enhancing immune responses against mutable pathogens. escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. the aim of this study was the optimization of multi-epitopic hiv-1 tat/pol/gag/env ...

Journal: :iranian journal of parasitology 0
p shahbazi p shayan e ebrahimzadeh s rahbari

background: cryptosporidium parvum is a parasitic protozoan that functions as important causa­tive agent of diarrhea in human and animals. the host's immune response to surface anti­gens of c. parvum has been previously demonstrated. in this respect, the role of humoral immu­nity in the development of host protective immunity against this protozoon has been well demon­strated. methods: the effe...

2017
Wojciech Potocki Alessandro Negri Grażyna Peszyńska-Sularz Krzysztof Hinc Michał Obuchowski Adam Iwanicki

BACKGROUND Bacillus subtilis spores can be used for presentation of heterologous proteins. Two main approaches have been developed, the recombinant one, requiring modification of bacterial genome to express a protein of interest as a fusion with spore-coat protein, and non-recombinant, based on the adsorption of a heterologous protein onto the spore. So far only single proteins have been displa...

Journal: :The Journal of biological chemistry 1994
J A Rosenthal S H Hsu D Schneider L N Gentile N J Messier C A Vaslet E Hawrot

In order to explore the structure-function relationships of the curare mimetic alpha-neurotoxins we have constructed and cloned a synthetic gene for Bungarus multicinctus alpha-bungarotoxin which is expressed in Escherichia coli. The recombinant alpha-bungarotoxin is expressed as a fusion protein with alpha-bungarotoxin linked to the COOH-terminal end of the T7 Gene 9-encoded coat protein. Afte...

Journal: :iranian journal of basic medical sciences 0
hossein fahimi department of genetics, faculty of biological sciences, tarbiat modares university, tehran, iran 2 department of cellular and molecular biology, islamic azad university, pharmaceutical sciences branch (iaups), tehran, iran hossein allahyari department of immunology, faculty of medical sciences, tehran medical university, tehran, iran zuhair m hassan department of immunology, faculty of medical sciences, tarbiat modares university, tehran, iran majid sadeghizadeh department of genetics, faculty of biological sciences, tarbiat modares university, tehran, iran

objective(s): production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. the goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice. materials and methods: multiple amino acid sequences of different i...

Journal: :iranian journal of pharmaceutical research 0
hamid reza heidari department of pharmaceutical biotechnology, faculty of pharmacy, shahid beheshti university of medical sciences, tehran, iran mojgan bandehpour cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran department of biotechnology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran hossein vahidi department of pharmaceutical biotechnology, faculty of pharmacy, shahid beheshti university of medical sciences, tehran, iran jaleh barar research centre for pharmaceutical nanotechnology, tabriz university of medical sciences, tabriz, iran bahram kazemi shahid beheshti university of medical sciences hossein naderi-manesh department of biophysics, faculty of biological sciences, tarbiat modares university, tehran, iran

molecular farming has been considered as a secure and economical approach for production of biopharmaceuticals. human tnf related apoptosis inducing ligand (trail) as a promising biopharmaceutical candidate has been produced in different expression hosts. however, little attention has been paid to molecular farming of the trail in spite of numerous advantages of plant expression systems. theref...

Journal: :archives of clinical infectious diseases 0
mojgan bandehpour cellular and molecular biology research center, shahid beheshti university, m.c., tehran, ir iran narges abdali islamic azad university, research and science campus, tehran, ir iran farzaneh sadeghi department of biology, shahid beheshti university, m.c., tehran, ir iran kazem parivar islamic azad university, research and science campus, tehran, ir iran zarrin sharifnia cellular and molecular biology research center, shahid beheshti university, m.c., tehran, ir iran hossein ryahi department of biology, shahid beheshti university, m.c., tehran, ir iran

background brucellosis is an important cosmopolitan infection disease caused by organisms belonging to the genus brucella. the cgt gene (cyclic β-1, 2 glucan transporter gene) is a virulent factor in brucella genus. the present study was conducted with the aim of cloning and expression of brucella cgt gene. materials and methods brucella melitensis cgt gene was amplified from extracted chromoso...

Journal: :Journal of bacteriology 2009
Daniela Krajcíková Magda Lukácová Denisa Müllerová Simon M Cutting Imrich Barák

The capability of endospores of Bacillus subtilis to withstand extreme environmental conditions is secured by several attributes. One of them, the protein shell that encases the spore and is known as the coat, provides the spore with its characteristic resistance to toxic chemicals, lytic enzymes, and predation by unicellular and multicellular eukaryotes. Despite most of the components of the s...

زیبایی, سعید, حق‌پرست, ، علیرضا , دوستی, محمد , طهمورث‌پور, مجتبی , نصیری, ، محمدرضا ,

     Foot-and-mouth disease (FMD) is an extremely infectious and occasionally fatal viral disease with a rapid onset and a short course that affects cloven-hoofed animals and results in considerable financial losses. For this reason, availability of more reliable and efficient vaccines against Foot-and-mouth disease virus (FMDV) is felt as a necessity. The purpose of this study is to develop a ...

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