نتایج جستجو برای: recombinant coat protein
تعداد نتایج: 1310399 فیلتر نتایج به سال:
Display of proteins like feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater efficiency in the manufacturing of animal products and minimizing the environmental 5 impact of increased animal production. To deliver their full potential in the gut, feed enzymes must survive the ha...
objective(s):multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (dcs) are promising approaches for enhancing immune responses against mutable pathogens. escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. the aim of this study was the optimization of multi-epitopic hiv-1 tat/pol/gag/env ...
background: cryptosporidium parvum is a parasitic protozoan that functions as important causative agent of diarrhea in human and animals. the host's immune response to surface antigens of c. parvum has been previously demonstrated. in this respect, the role of humoral immunity in the development of host protective immunity against this protozoon has been well demonstrated. methods: the effe...
BACKGROUND Bacillus subtilis spores can be used for presentation of heterologous proteins. Two main approaches have been developed, the recombinant one, requiring modification of bacterial genome to express a protein of interest as a fusion with spore-coat protein, and non-recombinant, based on the adsorption of a heterologous protein onto the spore. So far only single proteins have been displa...
In order to explore the structure-function relationships of the curare mimetic alpha-neurotoxins we have constructed and cloned a synthetic gene for Bungarus multicinctus alpha-bungarotoxin which is expressed in Escherichia coli. The recombinant alpha-bungarotoxin is expressed as a fusion protein with alpha-bungarotoxin linked to the COOH-terminal end of the T7 Gene 9-encoded coat protein. Afte...
objective(s): production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. the goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice. materials and methods: multiple amino acid sequences of different i...
molecular farming has been considered as a secure and economical approach for production of biopharmaceuticals. human tnf related apoptosis inducing ligand (trail) as a promising biopharmaceutical candidate has been produced in different expression hosts. however, little attention has been paid to molecular farming of the trail in spite of numerous advantages of plant expression systems. theref...
background brucellosis is an important cosmopolitan infection disease caused by organisms belonging to the genus brucella. the cgt gene (cyclic β-1, 2 glucan transporter gene) is a virulent factor in brucella genus. the present study was conducted with the aim of cloning and expression of brucella cgt gene. materials and methods brucella melitensis cgt gene was amplified from extracted chromoso...
The capability of endospores of Bacillus subtilis to withstand extreme environmental conditions is secured by several attributes. One of them, the protein shell that encases the spore and is known as the coat, provides the spore with its characteristic resistance to toxic chemicals, lytic enzymes, and predation by unicellular and multicellular eukaryotes. Despite most of the components of the s...
Foot-and-mouth disease (FMD) is an extremely infectious and occasionally fatal viral disease with a rapid onset and a short course that affects cloven-hoofed animals and results in considerable financial losses. For this reason, availability of more reliable and efficient vaccines against Foot-and-mouth disease virus (FMDV) is felt as a necessity. The purpose of this study is to develop a ...
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