serological methods are commonly used methods for detection of viruses. preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies. in this research the gene encoding coat protein of a beet western yellows virus (bwyv) isolate from iran was amplified by pcr and was ligated into a bacterial expression vector (pet26b) to obtain pet-bwyv-cp cl...

background: the advent of recombinant dna technology has facilitated heterologous expression of proteins from various sources in different host systems including escherichia coli. if a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Background: Expression of virus coat protein (CP) in Escherichia coli often leads to production of partially folded aggregated proteins which are called inclusion bodies. Grapevine fanleaf virus (GFLV) is one of the most serious and widespread grapevine virus diseases around the world and in Iran.Objective: The main objective of this study was to find a s...

citrus tristeza virus (ctv) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in iran, as one of the major citr...

apple stem pitting virus (aspv) is one of the most common viruses of apple and pear trees. during 2012 to 2013, a total of 1053 symptomatic and asymptomatic leaf samples were collected from orchards in nine provinces of iran. aspv infection was detected by das-elisa and pcr in 54 samples (5.12%) from seven provinces. based on the geographical origin, 22 representative isolates were selected for...

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

background: transient and stable transformation of host plants are the common techniques to produce transgenic plants. however, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. while, transient gene expression is a quick method to produce recombinant proteins in plants.  objective: the main goal of the present study was to eval...

recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...