background: rotaviruses cause diarrhea in infants and young children worldwide. rotavirus outer capsid protein, vp7 is major neutralizing antigen that is important component of subunit vaccine to prevent rotavirus infection. many efforts have been done to produce recombinant vp7 that maintain native characteristics. we used baculovirus expression system to produce rotavirus vp7 protein and to s...

Selection of a system for successful recombinant protein production is important. The aim of this study wasto produce high levels of human interleukin-2 (hIL-2) in soluble form. To this end, the pET32a vector inEscherichia coli BL21 (DE3) was used as an expression system, since it was previously used for the productionof mouse IL-2 in soluble form. The results indicated that c...

background: infectious bursal disease virus (ibdv) causes a highly immunosuppressive disease in chickens and is a pathogen of major economic importance to the poultry industry worldwide. the vp2 protein is the major host-protective immunogen of ibdv and has been considered as a potential subunit vaccine against the disease. vp2 coding sequence was cloned in an inducible fungal vector and the pr...

helicobacter pylori infection is among the most prevalent infections in the world involving more than half the adult population. h. pylori infection results in active chronic gastritis, peptic ulcers and enhances the risk of gastric malignancies. it is of utmost importance to prevent h. pylori infection particularly in highly prevalent countries including iran. the urease holoenzyme produced by...

Mannheimia haemolytica is responsible for considerable economic losses to cattle, sheep, and goat industries in many parts of the world. This bacterium isone of the causative agents of shipping fever in cattle. Current vaccines against M. haemolytica are moderately efficacious since they do not provide complete protection against the disease. Production of a...

Background: Follicle stimulating hormone is a heterodimeric protein composed of two subunits, α and ß, which are linked noncovalently. The hypophysial gonadotropin FSH plays an important role in the regulation of oocyte maturation, and a key component for growth of ovulator follicles in ewes. Materials and Methods: This study seeks to clone and express the ovine follicle stimulating hormone sub...

Antibodies provide a suitable tool in fundamental research and their high affinity and specificity make them invaluable for diagnostic and therapeutic applications. A promising alternative to conventional antibodies are the heavy chain antibodies (VHH) of Camelidae having short length, high solubility and stability are preferred to other antibody derivatives. In this study, our goal was product...