Studies were conducted on the improvement of A. culicicola identification. This species is phenotypically very similar to A. veronii biotype sobria, A. sobria, and A. allosaccharophila. The sequences of 16S rDNA of A. culicicola isolates show the highest similarity with A. jandaei, A. veronii, and A. caviae. Digestion of 16S rDNA PCR product with AluI and MboI restriction endonucleases allowed ...

The determination of unknown DNA sequences around a known locus has important applications in molecular genetics, specifically in genomic walking and genome mapping. Several PCR-based methods have been reported to address this issue, but they often involve multiple, time-consuming steps. We have previously described a technique known as restriction site PCR (RS-PCR) that allows sequence acquisi...

A 14 kb maize DNA fragment carrying nuclear rRNA genes and spacer regions was isolated and characterised by restriction enzyme mapping. A complete 3020 bp long external spacer region was sequenced and revealed 9 tandemly arranged 200 bp long repeat units with high homology. The repeat units lie upstream from two prominent S1 mapping signals. The sequence of a typical repeat unit is compared to ...

Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the pa...

The present investigation was to study DNA polymorphism of insulin-like growth factor binding protein-3 (IGFBP-3) gene by PCR-RFLP in four Egyptian local sheep breeds. Genomic DNA was isolated from a total of 20 animals from four Egyptian breeds (five each) of sheep namely Rahmani, Ossimi, Awassi, and Barki. A fragment of IGFBP-3 gene, comprising of a part of exon 2, complete intron 2, exon 3, ...

We have developed a rapid method for the isolation of leptospiral chromosomal DNA which yields DNA of a purity suitable for restriction endonuclease analysis. A small volume (15 to 20 ml) of an exponentially growing culture of leptospires yielded 2 to 4 micrograms of chromosomal DNA. In a 1-day protocol, the DNA was isolated, restricted with endonucleases, and fractionated on an agarose gel. Ch...

PURPOSE To detect the genetic changes in adenovirus type 4 (Ad4) by DNA restriction endonuclease analysis during 1993-1995, when no epidemic outbreak of conjunctivitis occurred. METHODS We evaluated 16 Ad4 strains from patients with acute viral conjunctivitis at four eye clinics in Sapporo, northern Japan. Seven strains were obtained during the period from December 1993 through March 1994 (fi...

Since the 1960s, biological macromolecules have occupied an increasingly important role in evolutionary and systematic studies. These biological macromolecules, used as sources of molecular characteristics, are proteins and various types of DNA such as a nuclear genome, nuclear ribosomal DNA, mitochondrial genome, and chloroplast genome. Electrophoresis and immunological techniques are used for...

Tetrahymena is a ciliated protozoan which has two nuclei: a micronucleus, which maintains the genetic continuity of the cell, and the macronucleus which is derived from the micronucleus after sexual conjugation. A macronuclear DNA library was constructed to contain DNA ends. A probe containing C4A2 repeats which are known to be present at macronuclear DNA ends (1) was used to screen the library...

Isolates of herpes simplex virus which had previously been typed by restriction enzyme analysis were typed again with a commercial ELISA system using polyclonal antibodies. There was complete correlation between the two techniques. Although restriction is more precise and definitive, when typing only is required the simplicity of ELISA makes it the preferred technique.