نتایج جستجو برای: restriction enzymes

تعداد نتایج: 192232  

Journal: :iranian journal of basic medical sciences 0
reza shafiei department of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, iran bahador sarkari basic sciences in infectious diseases research center, shiraz university of medical sciences, shiraz, iran abdolali moshfe cellular and molecular research center, yasuj university of medical sciences, yasuj, iran

objective(s): fascioliasis is a zoonotic parasitic disease caused by liver fluke species of fasciola hepatica and fasciola gigantica. differentiation of these two species, based on their morphological characteristics, is difficult. the current study aimed to use pcr-rflp assay to distinguish between f. hepatica and f. gigantica , based on profiles of rflp, produced by effect of endonucleases on...

Journal: :iranian journal of microbiology 0
j a qasem department of applied medical sciences, college of health sciences, public authority for applied education and training-paaet, kuwait. m a al-fadhli department of medicine, infectious disease hospital, ministry of health, kuwait. m a saraya department of medicine, infectious disease hospital, ministry of health, kuwait. j thomas department of applied medical sciences, college of health sciences, public authority for applied education and training-paaet, kuwait.

background and objectives: varicella–zoster virus (vzv) is a human herpes virus that usually attacks young children and commonly causes chicken pox (varicella). following primary infection, a lifelong latent infection is established. the virus often reactivates during adulthood or senesces to cause shingles (zoster). little is known regarding the genotypes of varicella in kuwait. the aim of thi...

Journal: :Journal of bacteriology 2015
Louise Roer Frank M Aarestrup Henrik Hasman

The rapid evolution of bacteria is crucial to their survival and is caused by exchange, transfer, and uptake of DNA, among other things. Conjugation is one of the main mechanisms by which bacteria share their DNA, and it is thought to be controlled by varied bacterial immune systems. Contradictory results about restriction-modification systems based on phenotypic studies have been presented as ...

Journal: :Cold Spring Harbor Protocols 2021

2010
Tiratha Raj Singh

UNLABELLED Restriction endonucleases are indispensable tools in molecular biology and biotechnology. Type II restriction endonucleases are part of restriction modification systems. DNA fragment extraction and restriction mapping are the basis for several biotechnological activities. WebFARM is a server application for identifying restriction endonuclease recognition sites and to give informatio...

Journal: :International journal of food microbiology 1994
A Querol E Barrio D Ramón

Using mitochondrial DNA restriction endonuclease analysis, the dynamics of the natural Saccharomyces cerevisiae strains present in spontaneous wine fermentations have been studied. We observed a sequential substitution of Sacch. cerevisiae strains along fermentation agreeing with different fermentation phases. When the restriction pattern's similarity (measured as the fraction of shared restric...

Journal: :iranian journal of parasitology 0
mb ghavami p rahimi a haniloo sn mosavinasab

background: to identify the fasciolid species by morphometric and molecular methods in zanjan, north­west of iran. methods : adult fasciola worms (n=584) were obtained from cattle and sheep in zanjan slaughterhouse in 2007. living flukes were washed, then worms' images were taken by 3ccd camera and finally apical zone of each worm was obtained. morphometric values such as body length, body widt...

Journal: :journal of medical microbiology and infectious diseases 0
mir mostafa ghamilouie dept. of parasitology, pasteur institute of iran, tehran zarrintaj valadkhani dept. of parasitology.12th farvardin. pasteur institute of iran fariba khoshzaban shahed university, tehran, iran

introduction: acanthamoeba is an opportunistic protist, which is ubiquitously distributed in the environment. infection with acanthamoeba spp. poses threat to human health, such as, acanthamoeba keratitis (ak) that is a vision-threatening infection of the cornea. this study aimed to identify the species of acanthamoeba strains isolated from cornea of keratitis patients by polymerase chain react...

2017
Giedrius Sasnauskas Giedrė Tamulaitienė Gintautas Tamulaitis Jelena Čalyševa Miglė Laime Renata Rimšelienė Arvydas Lubys Virginijus Siksnys

Type II restriction endonucleases (REases) form a large and highly diverse group of enzymes. Even REases specific for a common recognition site often vary in their oligomeric structure, domain organization and DNA cleavage mechanisms. Here we report biochemical and structural characterization of the monomeric restriction endonuclease UbaLAI, specific for the pseudosymmetric DNA sequence 5'-CC/W...

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