نتایج جستجو برای: somatic embryos
تعداد نتایج: 84378 فیلتر نتایج به سال:
The article summarizes results of studies concerning: 1/ qualitative evaluation of pig nuclear donor cells to somatic cell cloning, 2/ developmental potency of sheep somatic cells to create chimera, 3/ efficient production of chicken chimera. The quality of nuclear donor cells is one of the most important factors to determine the efficiency of somatic cell cloning. Morphological criteria common...
A cotransformation system using somatic embryos was developed to produce grapevines free of selectable marker genes. This was achieved by transforming Vitis vinifera L. "Thompson Seedless" somatic embryos with a mixture of two Agrobacterium strains. The first strain contained a binary plasmid with an egfp gene of interest between the T-DNA borders. The second strain harbored the neomycin phosph...
Pyruvate kinase (EC 2.7.1.40) is one of the key regulatory enzymes controlling glycolysis in plants and animals. It catalyzes the irreversible formation of pyruvate and ATP from phosphoenolpyruvate and ADP. In plants, pyruvate kinases can be either heteroor homotetramers depending on the source of the isozyme (Plaxton, 1989). Pyruvate kinase enzymes have been purified or partially purified from...
Plant regeneration and somatic embryogenesis through interspecific hybridization among different Carica species were studied for the development of a papaya ringspot virus-resistant variety. The maximum fruit sets were recorded from the cross of the native variety C. papaya cv. Shahi with the wild species C. cauliflora. The highest hybrid embryos were recorded at 90 days after pollination and t...
The propagation of oil palm seeds through tissue culture and somatic embryogenesis is a preferred method due to its ability produce entire plants without the need for gamete fusion. While embryo proliferation typically performed on solid media, this approach less efficient than liquid which both more cost-effective can lead better results. Thus, study aimed determine most effective combination ...
Ficus lyrata is a popular ornamental foliage plant with unique violin- or guitar-shaped green leaves. In our previous study, grapevine gene VvMybA1 was introduced into F. via Agrobacterium-mediated transformation, which resulted in the availability of purple-leaved plants. Since transcription factor, regeneration transgenic plants might potentially produce variants multicolored The objective th...
A simple and efficient protocol for recurrent somatic embryogenesis and plant regeneration is one of the prerequisites for genetic improvement of guava. An efficient reproducible regeneration somatic embryogenesis protocol was developed in four genotypes of Psidium guajava L. using immature zygotic embryo as starter explant. Somatic embryogenesis induction was obtained on MS basal medium supple...
Node, internode, and leaf explants of Populus deltoides Bartr. ex Marsh. × Populus deltoides Bartr. ex Marsh. hybrid poplar clone (89 M 066) tissue culture regenerated plantlets were used for direct and indirect somatic embryogenesis. Direct somatic embryos were differentiated on node, internode and leaf explants on Murashige and Skoog basal medium (MS) supplemented with N 6 -Benzyladenine (BA)...
In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their developmental capacity by investigating the effect of osmolarity and macromolecules in the culture medium on the in vitro developmental capacity. Hybrid embryos produced by the electrofusion of fet...
background: genome instability is a main cause of chromosomal alterations in both somatic and germ cells when exposed to environmental, physical and chemical genotoxicants. germ cells especially spermatozoa are more vulnerable to suffering from dna damaging agents during spermatogenesis and also more potent in transmitting genome instability to next generation. methods: to investigate the effec...
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