نتایج جستجو برای: somatic embryos

تعداد نتایج: 84378  

ژورنال: علوم آب و خاک 2007
امید کرمی, , علی دلجو, , محمود اثنی عشری, ,

In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30...

ژورنال: علوم آب و خاک 2008
محمودی پور, عقیل , دلجو, علی , کرمی, امید ,

In vitro regeneration of two cultivars of carnation, namely, ‘Nelson’ and ‘Impulse’ was studied through direct somatic embryogenesis. Somatic embryos were formed directly on petal explants cultured on Murashige and Skoog (MS) medium containing different concentrations (0.2, 0.5, 1, 2, 4, 6 mg 1-1) of picloram. Maximum embryogenesis was obtained with 1 and 2 mg/l picloram. Globular shaped embryo...

امید کرمی, , علی دلجو, , محمود اثنی عشری, ,

In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30...

محمودی پور, عقیل , دلجو, علی , کرمی, امید ,

In vitro regeneration of two cultivars of carnation, namely, ‘Nelson’ and ‘Impulse’ was studied through direct somatic embryogenesis. Somatic embryos were formed directly on petal explants cultured on Murashige and Skoog (MS) medium containing different concentrations (0.2, 0.5, 1, 2, 4, 6 mg 1-1) of picloram. Maximum embryogenesis was obtained with 1 and 2 mg/l picloram. Globular shaped embryo...

Journal: :به نژادی نهال و بذر 0
علی عبادی a. ebadi university college of agriculture and natural resources, university of tehran, karaj, iran.، پردیس کشاورزی و منابعطبیعی دانشگاه تهران، کرج امیر جمال محمود a. jamal mahmood مسعود میرمعصومی m. mirmasoomi منصور امیدی m. omidi

to produce embryogenic callus and somatic embryos, tendril explants were collected and cultured in ms medium supplemented with various concentrations of hormones. after one month most of explants produced callus. the highest percent of embryogenic callus obtained in ms medium supplemented with 4.5 µm bap and 1µm 2,4-d. production of somatic embryos was depended on genotype and medium. yaghouti ...

Forouzanfar M Gourabi H Hajian M Hosseini M Jafarpour F Ostadhosseini S Shahverdi AH

Background: At the outset of the somatic cell nuclear transfer (SCNT) process, the chromatin structure of the somatic cell which governs its state of differentiation undergoes dramatic changes, called reprogramming, and is compelled back to the embryonic stage. However, the overall epigenetic makeup of the resultant cloned embryos has been acknowledged far different from the fertilized embryos....

ژورنال: علوم آب و خاک 2009
ابراهیمی, مرتضی, خیام نکویی, سیدمجتبی, کدخدایی, سعید,

Somatic embryogenesis is affected by several factors. In this research project, we studied the effect of explant size, wounding and desiccation treatments on somatic embryogenesis and their conversion into plantlet among three genotypes of soybean. The explants were sampled from immature embryos of soybean in three different sizes (3, 5 & 7 mm) with wounding treatment on half of each, and then ...

ابراهیمی, مرتضی, خیام نکویی, سیدمجتبی, کدخدایی, سعید,

Somatic embryogenesis is affected by several factors. In this research project, we studied the effect of explant size, wounding and desiccation treatments on somatic embryogenesis and their conversion into plantlet among three genotypes of soybean. The explants were sampled from immature embryos of soybean in three different sizes (3, 5 & 7 mm) with wounding treatment on half of each, and then ...

Journal: :journal of agricultural science and technology 2016
m. a. sheikh beig goharrizi a. dejahang m. tohidfar a. izadi darbandi c. nestor

somatic embryos of persian walnut were transformed with two strains of agrobacterium tumefaciens i.e. lba4404 and c58, and two plasmids, namely, pbi121 with nptii and gus genes for improving the transformation protocol, and p6u-ubi-fvti plasmid containing the hpt and fld genes. the transformation frequency was 10%. pcr and rt-pcr analysis proved the presence and expression of the genes. the tra...

Journal: :journal of sciences islamic republic of iran 0

somatic embryogenesis was initiated in c. sativus l. from shoot meristems on ls medium containing bap (2×10-5 m)+naa (2×10-5 m). various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. matured embryos coul...

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