Block copolymers were recently used to promote gene delivery in various tissues. Using a plasmid encoding a food allergen, bovine beta-lactoglobulin (BLG), we studied the effects of block copolymers on gene expression levels and primary immune response and on further induced allergy. Block copolymers (i.e., Tetronic 304, 908, and 1107) and various quantities of DNA were injected into the tibial...

Granulocyte-macrophage colony-stimulating factor (GMCSF) is an important cytokine for the generation and propagation of antigen-presenting cells and for priming a cellular immune response. We report here that use of recombinant human GM-CSF (rhGM-CSF), administered as an adjuvant in a peptide-based vaccine trial given monthly by intradermal injection, led to the development of a T-cell and anti...

Background & Objective: DNA vaccines as a new generation of vaccines require adjuvant to improve vaccine immunogenicity; adjuvants can also increase the DNA vaccine efficacy. In this study, the effects of the host’s interferon-inducible Mx protein as bio adjuvant and conventional alum adjuvant were evaluated.   Materials & Methods: The BALB/c mice were immunized by different prime-boost strate...

This study was performed in order to define the cellular immune response of 12 recovered cutaneous leishmaniasis subjects to different soluble antigens of the amastigote form of Leishmania major. A soluble leishmanial antigen preparation (SLA) derived from highly-purified amastigotes from infected nude mice was fractionated by Mono Q column using Fast Protein Liquid Chromatography (FPLC) s...

DNA vaccines induce humoral and cellular immune responses in animal models and humans. To analyze the immunogenicity of the severe acute respiratory syndrome (SARS) coronavirus (CoV), SARS-CoV, spike DNA vaccine and the immunoregulatory activity of interleukin-2 (IL-2), DNA vaccine plasmids pcDNA-S and pcDNA-IL-2 were constructed and inoculated into BALB/c mice with or without pcDNA-IL-2 by usi...

Background: Tuberculosis (TB) remains an important health problem throughout the world. Despite its significance in public health, mechanisms of protective immunity against Mycobacyerium Tuberculosis in humans have not yet been understood. Objective: To evaluate cell mediated immune response against purified Ag 85, PPD and Phytohemagglutinin (PHA) in patients with tuberculosis and healthy tuber...

background: tuberculosis (tb) remains an important health problem throughout the world. despite its significance in public health, mechanisms of protective immunity against mycobacyerium tuberculosis in humans have not yet been understood. objective: to evaluate cell mediated immune response against purified ag 85, ppd and phytohemagglutinin (pha) in patients with tuberculosis and healthy tuber...

AIM to describe non-spesific and specific immune response profile in Indonesian thalassemia major with and without splenectomy. METHODS this study was held at Thalassaemia Centre, Cipto Mangunkusumo Hospital Jakarta on September 2013-February 2014. A comparative cross sectional study was conducted in healthy, thalassemia major aged more than 12 year and seronegative HIV. They were matched in ...