General biochemistry detection on paper-based microanalytical devices (PADs) uses pipette titration. However, such an approach is extremely time-consuming for large-scale detection processes. Furthermore, while automated methods are available for increasing the efficiency of large-scale PAD production, the related equipment is very expensive. Accordingly, this study proposes a low-cost method f...

The redox potential of deazariboflavin has been determined for pH values from 5.5 to 9.2 by equilibration with riboflavin and lumiflavin 3-acetate. The position of the equilibrium with riboflavin was measured spectrophotometrically and fluorimetrically; the equilibrium potential with lumiflavin 3-acetate was measured spectrophotometrically and potentiometrically. The Em7 for deazariboflavin was...

Zinc is one of the most widespread metal ions found in biological systems. Of the expected 3000 zinc proteins in the human proteome, most contain zinc in structural sites. Among these structures, the most important are zinc fingers, which are well suited to facilitate interactions with DNA, RNA, proteins and lipid molecules. Knowledge regarding their stability is a critical issue in understandi...

Calorimetric titrations of glycogen phosphorylase b (EC 2.4.1.1) from rabbit muscle with its allosteric activator, AMP, have been carried out at 25 “C and pH 6.9 in three different buffer systems (glycylglycine, glycerophosphate, and tris(hydroxymethy1)aminomethane). Calorimetric curves of biphasic nature were originally obtained. This biphasic behavior was always concomitant with: (a) a depen...

Arbutin is the main active ingredient in many herbal medicinal products that have diuretic, antimicrobial, bactericidal, and antioxidant effects. Many of these are mixtures different substances. Therefore, approaches to quality control HMPs can vary significantly. The aim study was compare arbutin assay procedures used arbutin-containing products. Materials methods. Samples following were study...