Cartilage is a tissue that derives its unique mechanical and biological properties from the combination of relatively few cells and a large amount of a complex extracellular matrix. Furthermore, cartilage tissue is comparatively slow to respond to changes or harmful influences. To date, the optimal generation and long-term maintenance of cultured human articular cartilage for in vitro testing o...

Background : Meningitis is more common during the neonatal period than other periods of life and has a prevalence of one in 1000 live births. The similarity of its clinical manifestations with other infectious diseases makes it difficult to diagnose. The aim of the current study was to determine the relationship between age, sex, clinical signs and symptoms and laboratory test results of ne...

Long-term culture of primary neonatal rat cardiomyocytes is limited by the loss of spontaneous contractile phenotype within weeks in culture. This may be due to loss of contractile cardiomyocytes from the culture or overgrowth of the non-cardiomyocyte population. Using the mitochondria specific fluorescent dye, tetramethylrhodamine methyl ester perchlorate (TMRM), we showed that neonatal rat ca...

The article describes the term “project culture” based on the viewpoints of several authors and ideas given in different sources. Based on the existing views and opinions the author tries to define the term “project culture” from the point of view of pedagogic. Moreover, the author emphasizes the advantages of reflection of project culture in the image of the individual under current conditions...

Effect of short-term exposure to nerve growth factor (NGF) on neuron survival of superior cervical ganglion (SCG) transplanted into the third ventricle in rats and that on neurite outgrowth of SCG neurons in a tissue culture system were examined. Because SCG taken from 3 week-old rats exhibited a high survival rate in transplantation and they were highly sensitive to NGF in culture, they were u...

Background: Activation of bone morphogenetic protein 4 (BMP4) signaling pathway in embryonic stem (ES) cells plays an important role in controlling cell proliferation, differentiation, and apoptosis. Adverse effects of BMP4 occur in a time dependent manner; however, little is known about the effect of different time exposure of this growth factor on cell number in culture media. In this study, ...