The distribution of dopamine-containing processes in the striatum of fetal and neonatal cats was studied by immunohistochemical and glyoxylic acid histofluorescence methods and compared to the distribution of acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7) observed by thiocholine histochemistry in the same or serially adjoining sections. Both methods for demonstrating the dopam...

marek’s disease (md) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. the present study was an attempt to use polymerase chain reaction (pcr) tooptimize a rapid and reliable assay for detection of mdv genome. detection of serotype 1 of mdv (mdv-1)was confirmed by presence of a 200 bp dna fragment as a pcr product. differentiation of mdv-1 ...

the opportunistic pathogen c.albicans is able to cause disseminated infections in immunocompromised patients. microbiological methods for the diagnosis of invasive candidiasis have many problems including low sensitivity, requirement to invasive clinical sampling such as biopsies or multiple blood cultures and need to expertise laboratory stuff. since pcr has proven to be a powerful tool in the...

background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certificat...

conclusions the results of the present study showed that in comparison with previously published primer sets, p. aeruginosa infection can be diagnosed with higher sensitivity and specificity by the conventional pcr assay using the newly designed primers. it was also shown that the results of the pcr assay on clinical samples of severe infections became positive much earlier than the results of ...

plagiarism refers to “adopting someone else’s words, work or ideas and passing them off as one’s own”. it is potentially considered as the most prevalent form of scientific dishonesty discovered in research papers. the present review aims to provide a thorough account of plagiarism to build awareness about all dimensions of plagiarism.the key words “plagiarism”, “types”, “detection” and “conseq...

easily degradating and various isomeric forms of rapamycin (sirolimus) face the determination of this compound to many challenges. in this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (rp-hplc) method for rapamycin. separation was performed on a c8 column (mz, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

a gradient reversed-phase high performance liquid chromatography (hplc) method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (gnrh) antagonistic activity used in infertility treatment. the hplc method, which is used to determine cetrorelix in bulk and pharmaceutical dosage forms, was validated per ich guidelines. the chromatograph...