Objective: To develop and validate a stability-indicating reversed phase high performance liquid chromatography (RP-HPLC) assay for the determination of phenytoin in bulk and pharmaceutical dosage forms. Methods: A HPLC instrument incorporating aZorbaxC-18 analytical column (250x4.6 mm, 5μm particles) with a mobile phase comprising acetonitrile: water in the ratio 50:50 (%v/v) was employed for ...

two multivariate calibration methods are compared for the simultaneous chromatographic determination and separation of sulfamethoxazole (smx) and phthalazine (phz) by high performance liquid chromatography (hplc). multivariate calibration techniques such as classical least squares (cls) and inverse least squares (ils) were introduced into hplc to determine the quantification by using uv detecto...

Recently a new detection method, based upon aerosol charging (the charged aerosol detector (CAD)) has been introduced as an alternative to evaporative light-scattering detector (ELSD), chemiluminescent nitrogen detector and refractive index detector for detection of non-ultraviolet and weakly ultraviolet active compounds and for UV-absorbing compounds in the absence of standards. The content of...

Abbreviations: FVIII/vWF: Complex of Factor VIII and von Willebrand Factor; MW: Molecular Weight; NDF: Number of Degree of Freedom; S/D: Solvent/Detergent; LEV/LEVs: Virus (es) Enveloped by Lipid Membrane; NLEV/NLEVs: Non-enveloped Virus (es); IFA method: Method of Immune-fermental Analysis; FITC: Fluorescein Isothiocyanate; RT-PCR method: Real-time PCR Method; TCID50: 50% Tissue Culture Infect...

In the present study, various mixtures of aqueous ethanol and acetone solutions were utilized to extract anthocyanins from eggplant peel by a discontinuous process to obtain a natural red-purple colorant. First, the most appropriate combination for extraction solvent was selected as ethanol 70 wt in water acidified with acetic acid 3 wt, which was determined by analysis of extraction yields and...

Crocins in commercial liquid saffron extracts (Saffr’activ®) were identified using high-performance chromatography (HPLC) with a diode array detector (DAD) and mass spectrometry (MS). The impact of storage on the qualities extract studied HPLC-DAD-MS by exposing trans-4-GG crocin to environmental factors. Light temperature induced degradation after only one week. Trans-4-GG was totally hydrolyz...

Two multivariate calibration methods are compared for the simultaneous chromatographic determination and separation of Sulfamethoxazole (SMX) and Phthalazine (PHZ) by High Performance Liquid Chromatography (HPLC). Multivariate calibration techniques such as Classical Least Squares (CLS) and Inverse Least Squares (ILS) were introduced into HPLC to determine the quantification by using UV d...

Ultraviolet radiation spectral irradiance was measured at different altitudes on horizontal and tilted planes in different azimuth directions on cloudless days in Austria, in March 2010, within the Impact of Climatic and Environmental factors on Personal Ultraviolet Radiation Exposure project framework. The presented results demonstrate variations of the UVB, UVA and biologically effective spec...