Purified total genomic DNA labeled with photobiotin was used as a probe for the identification and differentiation of strains of Echinococcus granulosus and to distinguish between the morphologically identical eggs of 2 taeniid species. Such biotinylated probes are easily prepared and can be utilized effectively for rapid screening and identification of taeniid isolates by dot and Southern blot...

One of the most prevalent lesions in DNA is the apurinic/apyrimidinic (AP) site, which is derived from the cleavage of the N-glycosyl bond by DNA glycosylase or by spontaneous depurination. AP sites are repaired by AP endonucleases during the process of base excision repair; however, an imbalance in this DNA repair system may cause mutations as well as cell death. We have established a sensitiv...

A DNA probe is described for the diagnostic and taxonomic identification of the North American cattle pathogen Leptospira interrogans genotype hardjo-bovis. The probe is specific for this genotype and does not hybridize to genomic DNA of any other leptospire pathogen commonly found in North America.

Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was ...

PoZyrnorphisrn. A multiallelic dinucleotide repeat (CA-microsatellite) polymorphism in genomic DNA of rainbow trout ( Oncorhynchus mykiss Walbaum) was detectable by polymerase chain reaction (PCR) and designated FGT2. Source and Description. Sequence was obtained by conventional dideoxy methods using T7 DNA polymerase. The template was isolated from a library of sizeselected (150 to 400 bp) Sau...

PoZymorphism. A multiallelic dinucleotide repeat (CA-microsatellite) polymorphism in genomic DNA of rainbow trout ( Oncorhynchus mykiss Walbaum) was detectable by polymerase chain reaction (PCR) and designated FGTS. Source and Description. Sequence was obtained by conventional dideoxy methods using T7 DNA polymerase. The template was isolated from a library of sizeselected (150 to 400 bp) Suu3A...

The streptomycin (SM) gene cluster was investigated for its distribution in streptomycetes by Southern hybridization using nick-translated DNA probes, which were isolated from the SM-6-phosphotransferase (SPH) and amidinotransferase (ADT) regions of the SM gene cluster of Streptomyces griseus SS-1198. Bgl II-digested genomic DNAs from SM-producing strains of S. griseus yielded the same size fra...

Fiber-based biosensors enable a new approach in analytical diagnostic devices. The majority of textile-based biosensors, however, rely on colorimetric detection. Here woven biosensor that integrates microfluidics structures combination with an electroanalytical readout based thiol-self-assembled monolayer (SAM) for Nucleic Acid Amplification Testing, NAATs is shown. Two types fiber-based electr...