Surface components of virulent and attenuated Leptospira interrogans serovar grippotyphosa were compared by using Triton X-114 solubilization and phase partitioning, immunoprecipitation of intact organisms, and freeze-fracture electron microscopy. Removal of the leptospiral outer membrane by using 0.1% Triton X-114 was demonstrated by whole-mount electron microscopy and by essentially complete ...

The prevalence of obesity is high in older persons and recent trends show a rapid increase in this prevalence. Results from observational and intervention studies (i.e. weight loss studies) show the strong negative impact of obesity on functional status in old age. There are different potential pathways through which obesity may lead to functional decline in older persons. Furthermore, the pres...

Introduction Hydrocolpos is an uncommon congenital disorder consisting of cystic dilatation of the vagina caused by obstruction of the distal genital tract, resulting in retained fluid. If there is also associated uterine enlargement, the term hydrometrocolpos is applied. Vaginal obstruction may result from a high vaginal septum, varying degrees of vaginal atresia, cloacal malformations, or an ...

Fungicide resistance in dollar spot caused by Sclerotinia homoeocarpa is a common problem on golf courses in North America (Figure 1) (2), and managing fungicide resistance is a complex and controversial topic (1,4). With dollar spot, the question of whether to use the same fungicide until resistance develops or to rotate different chemistries and tank mixtures has not been defi nitively answer...

Cloud point extraction was successfully applied to the preconcentration of phenothiazine derivatives, such as pericyazine (PC), chlorpromazine (CP) and fluphenazine (FUL), for gas chromatography (GC). Phenothiazine derivatives were separated from surfactants by passing the surfactant-rich phase through a cation exchange column after cloud point extraction, permitting the determination of the ph...