In vitro gene chemical synthesis is a powerful tool to improve the expression of gene in heterologous system. In this study, a two-step gene synthesis strategy that combines an assembly PCR and an overlap extension PCR (AOE) was developed. In this strategy, the chemically synthesized oligonucleotides were assembled into several 200-500 bp fragments with 20-25 bp overlap at each end by assembly ...

BACKGROUND Together with the development of optical sensors, fluorometry is becoming an increasingly attractive tool for the monitoring of cultivation processes. In this context, the green fluorescence protein (GFP) has been proposed as a molecular reporter when fused to target proteins to study their subcellular localization or secretion behaviour. The present work evaluates the use of the GFP...

Die hoë voorkoms van tuberkulose in die Kaapstadgebied, soos aangegee deur die mediese gesondheidsbeamptes van die Kaapse Afdelingsraad en die Stadsraad, is rede tot kommer. In 1982 was die syfers vir Swartes in die omgewing van 1 400 tot 1 500 per 100 000 en vir Kleurlinge in die omgewing van 300 tot 340 per 100 000. Die beraan.de bevolking in die WesKaap gesondheidstreke is 1,6-miljoen. Met d...

Retinoids have many beneficial effects on dermatological applications. But, retinoids cause skin irritation. In this study, the safety of retinoids was clarified via both primary skin irritation test in rabbits and sensitization study using an integrated model for the differentiation of chemical-induced allergic and irritant skin reaction (IMDS) , an alternative method to sensitization test. Th...

Given the interval model of an n-venex, e-edge circle graph G. it is shown how to find a clique of G of maximum size 1 Cresp.. maximum weight) in o (nlogn+min[e,nllog(2nll)]) (resp., O(nlogn+min[n 2,eloglogn])) time. The best previous algoritfuns required. respectively, Ben2) and O(n 2+eloglogn) time. An O(nlogn+dn) time and space algorithm that finds an independent set of maximum weight for th...