We designed a protein ladder (hereafter referred to as "Mega-tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder ...

After 20 years of a static clinical ladder program at our institution, the clinical ladder program was completely redesigned using a staff nurse-led shared governance structure to re-envision the program as an innovative, staff leadership model to meet our organizational nursing mission and vision strategic plans around retention and professional development. The literature demonstrated a lack ...

C = ladder network stage capacitance E = difference operator L = ladder network stage inductance ‘ = number of ladder network stages m = voltage measurement node n = ladder network node identifier, 0 n ‘ Rt = termination resistance s = Laplace variable V t = transmission line driving signal ~ Vn = Laplace transform of the voltage at any node n x = length of transmission line min = smallest wave...

Absrracr-The low sensitivity property of lossless discrete integrator (LDI) low-pass ladder filters is shown to be preserved in lossless discrete differentiator (LDD) high-pass ladder filters 111. The exact design method for LDI ladder filters given in [Z] is further developed by introducing a set of closed-form design formulas for digital ah-pole Chebyshev transfer functions. A new technique f...

INTRODUCTION Pemphigus and bullous pemphigoid (BP) are identified by autoantibodies (abs) against desmoglein 1, 3 (DSG1/3) and BP180/BP230, respectively. A novel mosaic to indirect immunofluorescence (IIF) using purified BP180 recombinant proteins spotted on slide and transfected cells expressing BP230, DSG1, DSG3 is available. The commercial (IgG detection) and modified (IgG4 detection) mosaic...

A differential poset is a partially ordered set with raising and lowering operators U and D which satisfy the commutation relation DU−UD = rI for some constant r. This notion may be generalized to deal with the case in which there exist sequences of constants {qn}n≥0 and {rn}n≥0 such that for any poset element x of rank n, DU(x) = qnUD(x)+rnx. Here, we introduce natural raising and lowering ope...

A link between matroid theory and p-branes is discussed. The Schild type action for p-branes and matroid bundle notion provide the two central structures for such a link. We use such a connection to bring the duality concept in matroid theory to p-branes physics. Our analysis may be of particular interest in M-theory and in matroid bundle theory.

A graph G∗ is 1-edge fault-tolerant with respect to a graph G, denoted by 1-EFT(G), if every graph obtained by removing any edge from G∗ contains G. A 1-EFT(G) graph is optimal if it contains the minimum number of edges among all 1-EFT(G) graphs. The kth ladder graph, Lk , is defined to be the cartesian product of the Pk and P2 where Pn is the n-vertex path graph. In this paper, we present seve...