diversity of durum wheat (triticum turgidum ssp. durum) germplasm was evaluated using simple sequence repeat (ssr) markers. one hundred and forty durum wheat genotypes of diverse origin (belonging to ten countries) were assessed using 48 selected ssrs with whole genome coverage. a total of 245 allelic variants were detected at 37 polymorphic ssr loci, ranging from two to seventeen per locus wit...

in an attempt to characterize theileria parva parasites circulating in south sudan cattle , polymerase chain reaction (pcr)-based assays were carried out using four single copy encoding antigen genes p104, pim, p150 and p67 in addition to one microsatellite ms321. a total of 20 bovine dna samples from two locations in south sudan were included in this study, in addition to two references strain...

Broadly applicable polymorphic genetic markers are essential tools for population genetics, and different types of markers have been developed for this purpose. Microsatellites have been employed as particularly polymorphic markers for over 20 years. However, PCR primers for microsatellite loci are often not useful outside the species for which they were designed. This implies that a new set of...

Microsatellites have proven to be very useful genetic markers for studies of kinship, parentage, and gene mapping. If microsatellites are conserved among species, then those developed for one species can be used on related species, which would save the time and effort of developing new loci. We evaluated conservation of 27 trinucleotide loci that were derived from 2 species of Polistes wasps in...

Ten polymorphic microsatellite loci for the obligate biotrophic, oomycete pathogen of tobacco, Peronospora tabacina, were identified from a small insert genomic library enriched for GT motifs. Eighty-five percent of the 162 loci identified were composed of dinucleotide repeats, whereas only 4% and 11% were tri-and tetra-nucleotide repeats respectively. About 82% of all the microsatellites were ...

Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...

We describe the isolation and characterization of 11 polymorphic microsatellite loci from the recently discovered fruit fly pest, Bactrocera invadens. The polymorphism of these loci was tested in individual flies from two natural populations (Sri Lanka and Democratic Republic of Congo). Allele number per locus ranged from three to 15 and eight loci displayed a polymorphic information content gr...

honeybee is a pollinator that has prominent role in agriculture products and nourishment for human. so, the most number of studies like genetic improvement were done on honeybee. recent advances in knowledge of molecular genetics of bee emphasize the potential for using genetic markers to select favored traits. this research was done to determine polymorphism of loci of first foraging age (aff)...