Background & Aims: Aflatoxin M1 is one of the most important toxins present in milk and the product of aflatoxin B1 metabolism in liver microsomes. The aim of this project was to study the aflatoxin M1 adsorption ability of polystyrene-hydroxyapatite nanocomposite. Methods: First, the probability of aflatoxin M1 adsorption by the nanocomposite was evaluated us...

Profiles of volatile metabolites of 150 urine samples from normal individuals and 40 samples from subjects with diabetes mellitus have been studied by gas chromatography and mass spectrometry. The technique involves adsorption of the urinary headspace volatiles on Tenax GC, heat desorption with helium, trapping on a cooled precolumn, and chromatography on 100-m X 0.50-mm i.d. nickel columns. In...

A new antifungal antibiotic, named ileumycin, was isolated from culture broth of streptomyces H 698-SY2, which was identified as S. lavendulae. The antibiotic was recovered from the culture filtrate by adsorption on Amberlite XAD-II and elution with aqueous methanol and was further purified by ion-exchange column chromatography on SE-cellulose and followed by partition chromatography on silica ...

A method for the isolation of liver microsomal cytochrome P-450 from phenobarbital-treated rats is described which is based on chromatography on 1-adamantane-carbonyl-aminohexyl-Sepharose 4B, subsequent ion exchange chromatography on DEAE-cellulose and detergent removal by adsorption on silica gel G. The method gives preparations with high electrophoretic homogeneity which correspond to a major...

Peptide mixtures, arising from such diverse sources as tissue extracts, urine or proteolysis, are usually fractionated and characterized by a combination of ion-exchange chromatography, gel-exclusion chromatography and/or electrophoresis. Appropriate combinations of these methods will normally yield a homogeneous product, but their costs, detection limits, analyses times and yields vary. The re...

Chromatography is a technique widely used in the purification of biopharmaceuticals, and generally consists several chromatographic steps. In this work, Hydrophobic Interaction (HIC) investigated as polishing step for therapeutic proteins. Adsorption mechanisms hydrophobic interaction chromatography are still not completely clear limited amount published data available. addition to new on adsor...

A brief review of charge-transfer (CT-) thromatography is presented. Hydrophilic gels such as Sephadex exhibit weak electron acceptor and donor prOperties due to the presence of matrix hydroxyls or matrix-bound water. Stronger O-adsorbents for chromatography in aqueous solutions are obtained by introduction of electron acceptor or donor substituents. The adsorption properties of such gels are d...