A healthy vaginal ecosystem has been shown to be protective against the acquisition of human immunodeficiency virus and gonorrhea, and women who are colonized with H(2)O(2)-producing lactobacilli are more likely to maintain a normal vaginal flora than women with lactobacilli that do not produce H(2)O(2). The purpose of this study was to formulate a testing medium that better supports the growth...

INTRODUCTION Sputum samples collected at a teaching hospital of Jodhpur, were processed at Desert Medicine Research Centre, Jodhpur. The aim was to assess the time required for primary isolation of Mycobacterium tuberculosis (Mtb) on locally prepared sheep blood agar slants as compared to Löwenstein-Jensen (LJ) medium. METHODS Equal volume of homogeneous inoculums prepared from smear positive...

The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye.

Halophilic bacteria were isolated from oil spill samples collected from West-coast of Goa. Bacteria were isolated from oil studded soil, salt marsh and offshore samples (A, A7, CSM, CB and CM) collected along the West coastline in Goa (India) i.e. Arambol beach, Calanguate beach, Candolim beach and Colva beach on Zobell Marine agar, R2A agar, Mannitol salt agar and Blood agar at temperature 22 ...

fungi associated with ascocarps of terfezia claveryi, tirmania nivea, t. pinoyi, picoa lefebvrei and p. juniperi in iran showed a wide range of genera and species in 94 samples collected during 2005–2009 from different regions including fars, systan and baluchestan, kerman, east azarbaijan, khuzestan, kermanshah and hormozgan provinces. thirty two species belonging to 21 genera were recovered f...

Four halotolerant fungal isolates originating from the saltwater Lake Urmia in Iran were selected during a screening program for salt resistance and α-amylase activity. The isolates were identified based on sequencing the ITS region and a part of the β-tubulin gene, as Penicillium chrysogenum (isolate U1; CBS 132820), Fusarium incarnatum (isolate U2; CBS 132821), and Penicillium polonicum (isol...

heavy metal pollution by natural factors is a world-wide phenomenon. release of large quantities of heavy metals without handling proper processes that could decrease the concentration of such a material is a hassle that makes strains resistant to these heavy metals apart from entering into human food chain. in this research, wastewater of four firms in guilan province such as foolad, risandegi...

background: glutathione (gsh) is a non-protein thiol compound, which plays an important role in the response to oxidative stress and nutritional stress. the aim of this study was to isolate indigenous s. cerevisiae strains capable of effectively produce gsh. methods: one hundred-twenty sweet fruit samples were collected. the strains were isolated on yeast glucose chloramphenicol (ygc) agar medi...

The MICs for 101 isolates of Neisseria gonorrhoeae obtained by Etest (AB-Biodisk, Solna, Sweden) and the agar dilution method on GC medium base supplemented with 1% Kellog's defined supplement (GCMB) were compared. The overall percent agreement (within 1 log2 dilution) between methods was greater than 97.9. The Pearson's correlation coefficients for penicillin, tetracycline, erythromycin, and c...