Gamma-glutamyltransferase activity in serum is shown to be competitively inhibited by the two substrates gamma-glutamyl-4-nitroanilide and glycylglycine. Awareness of this is of importance when one is choosing final reaction conditions for the assay of the enzyme. Gamma-glutamyltransferase probably acts by a "ping-pong bi-bi" kinetic mechanism, which fits with the double competitive substrate i...

Tyrosine aminotransferase purified from epimastigotes of Trypanosoma cruzi displays an additional activity of alanine aminotransferase, absent in all other tyrosine aminotransferases characterized so far. Since the parasite's genome contains a high number of copies of the tyrosine aminotransferase gene, we could not rule out the possibility that two very similar proteins, with changed specifici...

In 100 consecutive children aged below 18 years with confirmed typhoid fever, 29 had moderate hepatitis. Serum alanine amino transferase: lactate dehydrogenase (ALT: LDH) ratios of these 29 children at the time of hospitalization were compared with that of 29 children with acute viral hepatitis. The serum ALT: LDH ratio levels (expressed in multiples of upper limit of normal) was found to be le...

The present study was conducted to determine the hematobiochemical analysis of ostrich (Struthio camelus) with respect gender and progressive age. A total 40 apparently healthy ostriches both genders two age groups; young one (1 year 2.4 years: group A); adult (2.5 years 5 B) in equal number were used this study. Blood samples collected from wing vein maintained farms near Gogra Pakistan. For h...

OBJECTIVE: To investigate the serum antioxidant capacity and status of liver function parameters in Hepatitis B virus (HBV) C Virus (HCV) infected patients. METHODS: In this case-control study, fifty patients with HBV (28 male & 22 females), HCV females) age gender matched healthy controls were enrolled from May to August 2016. The was determined by measuring trolox equivalent oxidant (TEAC...

The aim of this research was to evaluate the role zinc supplementation on reduction heat stress in local lambs during hot season. In current experiment, twenty-seven were housed indoor individual pens (1.50 × 1.30m). Zinc added fresh drinking water three different levels namely T1 (0 mg Zn/day), T2 (36 Zn/day) and T3 (72 Zn/day). Serum (zinc; µmol/L), Alkaline phosphate (ALP; U/I), Alanine amin...

Background: Diethylnitrosamine (DEN) is a hepatotoxin whose metabolic activation by liver cytochromes P450 responsible for the necrosis, mutagenicity and carcinogenicity of cells. The purpose this study was to evaluate protective effects Calotropis procera roots bark against DEN induced hepatocellular damage in rats.
 Material Methods: Hepatoprotective activity ethanolic extract root were ...

In this investigation, the influence of doping graphene with silicon in the adsorption of alanine amino acid was inspected computationally. For this purpose, the structures of pure graphene, silicon doped graphene, alanine and 10 derived products of the alanine reaction with pure and silicon doped nano-adsorbents were optimized geometrically. Afterwards, the values of adsorption energy, formati...

PURPOSE Oxaliplatin displays a frequent dose-limiting neurotoxicity due to its interference with neuron voltage-gated sodium channels through one of its metabolites, oxalate, a calcium chelator. Different clinical approaches failed in neurotoxicity prevention, except calcium-magnesium infusions. We characterized oxalate outcome following oxaliplatin administration and its interference with cati...

Viral hepatitis B is a disease for which no curative treatment available to date. The objective of this study evaluate the effect Silybrum marianum and Lycopodium clavatum preparations on biological parameters viral follow-up.
 Material methods: This was longitudinal period 5 months. population consisted consenting chronic patients. A questionnaire used collect sociodemographic information...