An effective ultra-performance liquid chromatography coupled with the quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF/MS) method was developed for analysing the chemical constituents in rat plasma and urine after the oral administration of Rubia cordifolia L. extract. Under the optimized conditions, nine of 11 prototypes in rat plasma and four prototypes in urine were identified ...

The binding properties on alizarin to human serum albumin (HSA) have been studied for the first time using fluorescence spectroscopy in combination with UV-visible absorbance spectroscopy. The results showed that alizarin strongly quenched the intrinsic fluorescence of HSA through a static quenching procedure, and non-radiation energy transfer occurred within the molecules. The number of bindin...

Reference 1. Linero I, Chaparro O (2014) Paracrine Effect of Mesenchymal Stem Cells Derived from Human Adipose Tissue in Bone Regeneration. PLoS ONE 9(9): e107001. doi:10.1371/journal.pone.0107001 PMID: 25198551 Fig 3. Ad-MSC characterization. A. Osteogenic differentiation of Ad-MSC. Osteogenic differentiation was evidenced by the detection of calcium deposits with Alizarin Red staining. a. Con...