Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metallopro...

To provide a theoretical basis to improve the survival and growth rate and optimize diet of sheatfish (Silurus soldatovi), the activities of certain digestive enzymes and alkaline phosphatases were investigated during larval development of one-ten day old individuals. Results indicated that sheatfish larva (~ three days after hatching) had high levels of alkaline protease activity, which peaked...

While much attention has been given to marine microorganisms for production of enzymes, which in general are relatively more stable and active compared to those from plants and animals, studies on alkaline protease production from marine microorganisms have been very limited. In the present study, the alkaline protease producing marine bacterial strain SD8 isolated from sea muds in the Geziwo Q...

Piper chaba Hunter, a flowering vine of Piperaceae family, has been using for long time in south Asian country for culinary meat preparation. As stem and root of this plant has been using traditionally in fat rich meat preparation, the antioxidant and anticoagulation potential of this herb is a great interest of study and it was evaluated using cold and boiled water extract of dried and grinded...

A method for the quantitative estimation of arginine is included by Van Slyke (1911-12) in his procedure for the determination of the nitrogen distribution numbers of proteins. The amino acids present in the protein hydrolysate are divided into two groups by means of phosphotungstic acid. The portion precipitated by t,his reagent is referred to as the basic or diamino fraction and the portion n...

BACKGROUND & AIMS The pathogenesis of irritable bowel syndrome (IBS) remains only partially understood, and no specific or universally effective patient management procedure has been developed to date. Our study was designed to evaluate if colonic luminal serine-proteases may be a relevant pathophysiologic marker of IBS. METHODS Fecal samples of 38 IBS patients, 15 patients with ulcerative co...

The serine proteinase isolated from Thermomonospora fusca YX shows considerable thermal stability up to 80 °C, and progressive inactivation occurs at higher temperatures. Lyotropic salts affected the thermal stability of the enzyme at 85 °C, suggesting that disruption of hydrophobic interactions play an important role in the decreased thermal stability of the enzyme above 80 'C. Thermal stabili...

furin is a ca 2+ -dependent serine protease which cleaves proprotein substrates at the arg-xaa-(lys/arg)-arg    site to generate biologically active proteins. furin’s critical role in many cellular events associated with health disorders such as hiv, sars, anthrax, and influenza as well as cancer has made inhibitors of this enzyme as therapeutic targets. to this date, the most potent inhibitor ...

The aim of this study was optimization of the conditions for the production of alkaline protease from the Bacillus strains coded as L18, L21 and I18, which were isolated from our natural habitats. Additionally, the goal was also to optimize the production of alkaline protease from Bacillus sp. L21 in a new low-cost media formulation by employing design of experiments and response surface method...