Despite the fact that ionic liquid-based aqueous two phase systems (ATPSs) have been widely studied for extraction purposes, the adequacy of biodegradable organic salts as salting out agents has been left unexploited. In this study, we investigated the ability of sodium-based organic salts in the formation of ATPS in the presence of a common ionic liquid, [C4mim]BF4. In the pioneering aspect of...

Cephalexin is an important antibiotic. It is very significant to determine an appropriate method for the extraction of this valuable antibiotic for industrial applications. Aiming at developing an efficient method for the extraction of cephalexin, the partitioning of cephalexin has been evaluated in aqueous two-phase system, including cholinium chloride and potassium phosphate. The effect of th...

The surface properties of transfer RNA (tRNA) were analyzed using a poly(ethylene glycol)/dextran aqueous two-phase system (ATPS), where the surface net hydrophobicity (HFS) and the local hydrophobicity (LH) were evaluated based on the partition coefficient of tRNA in the ATPS. According to the evaluated HFS values, the surface of the tRNA molecule was hydrophilic at 20° -40 °C, and it became h...

Accurate disease diagnosis, patient stratification and biomarker validation require the analysis of multiple biomarkers. This paper describes cross-reactivity-free multiplexing of enzyme-linked immunosorbent assays (ELISAs) using aqueous two-phase systems (ATPSs) to confine detection antibodies at specific locations in fully aqueous environments. Antibody cross-reactions are eliminated because ...