This article shows the validity of Universal calibration concept in aqueous Size-exclusion chromatography for arabinogalactan and carboxymethylchitosans is presented. The electrostatic effects these polysaccharides during separation process were eliminated by utilizing a water-salt eluent as mobile phase, size-exclusion mechanism this method was realized.

∗Corresponding author. E-mail: [email protected] Abbreviations: AGP, Arabinogalactan protein; GPI-anchor, Glycosylphosphatidylinositol lipid anchor; HRGP, Hydroxyproline-rich glycoprotein; IT, Infection thread; NF, Nod-factor (Lipochitin oligosaccharide); LPS, lipolysaccharide; PRP, proline-rich protein; UDP, uridine diphosphate Colonization of host cells by rhizobium bacteria involves th...

Classical arabinogalactan proteins partially defined by type II O-Hyp-linked arabinogalactans (Hyp-AGs) are structural components of the plant extracellular matrix. Recently we described the structure of a small Hyp-AG putatively based on repetitive trigalactosyl subunits and suggested that AGs are less complex and varied than generally supposed. Here we describe three additional AGs with simil...

1. Background 1a Reticulocytes A number of density gradient strategies have been developed for the fractionation of human erythrocytes according to their age. As the cells age, so their density tends to increase; reticulocytes therefore have the lowest densities. Reticulocytes have frequently been partially purified on discontinuous gradients of arabinogalactan; the actual density range being q...

Pollen tubes elongate directionally in the extracellular matrix of pistil tissues to transport the male gametes from the apically located stigma to the basally located ovary for fertilization. The molecular mechanisms underlying directional pollen tube growth in the pistil are poorly understood. We have purified a glycoprotein, TTS, from tobacco stylar transmitting tissue, which supports pollen...

In spite of effective antibiotics to treat TB (tuberculosis) since the early 1960s, we enter the new millennium with TB, currently the leading cause of death from a single infectious agent, killing more than three million people worldwide each year. Thus an understanding of drug-resistance mechanisms, the immunobiology of cell wall components to elucidate host-pathogen interactions and the disc...