Colonization of nontypeable Haemophilus influenzae (NTHi) in nasopharynx causes respiratory tract disease. In 80% of clinical isolates, HMW proteins are the major adhesions and induce protective antibodies in the hosts. Therefore, it can be used as a vaccine candidate. The aim of this study is designing and cloning of the conserved regions of NTHi hmw1 core binding domain.In this study, the sta...

  The features of Helicobacter pylori adhesins, their interactions with their host counterparts, regulated and selective gene expressions are amongst the many clever strategies this microorganism undertakes to survive the otherwise sterile gastric milieu. The ingenious crafting of these interactions and the respective host reactions govern, in part, an array of consequences ranging from asympto...

We synthesized the first multifunctionalized phosphoinositide polyphosphate derivatives featuring a photo-removable protecting group (“cage”), photo-crosslinkable diazirine group, and terminal alkyne useful for click chemistry. demonstrate that lipid readily enter cells. After photo-crosslinking, cell fixation fluorescent tagging via chemistry, we determined intracellular location of before aft...

Understanding protein–ligand interactions in a cellular context is an important goal molecular biology and biochemistry, particularly for drug development. Investigators must demonstrate that drugs penetrate cells specifically bind their targets. Towards end, we present native mass spectrometry (MS)-based method analyzing uptake target engagement eukaryotic cells. This based on our previously i...