Background Resistance of Klebsiella pneumoniae to carbapenems is mainly associated with acquired carbapenem-hydrolyzing b-lactamases [1]. These b-lactamases can be metallo b-lactamases (IMP, VIM), expanded-spectrum oxacillinases (OXA-48), or Ambler class A enzymes (NMCA, IMI, SME, GES, and KPC) [1,2]. The most common class A carbapenemases in K. pneumoniae are the K. pneumoniae carbapenemases (...

The dissemination of antimicrobial resistance (AMR) is an escalating problem and a threat to public health. Comparative metagenomics was used to investigate the occurrence of antibiotic resistant genes (ARGs) in wastewater and urban surface water environments in Singapore. Hospital and municipal wastewater (n = 6) were found to have higher diversity and average abundance of ARGs (303 ARG subtyp...

Background and Aims: Klebsiella pneumoniae is a gram negative opportunistic pathogen of nosocomial infections that causes pneumonia, urinary tract infection (UTI), meningitis, septicemia and diarrhea. The emergence and spread of carbapenemase producing Klebsiella pneumoniae often cause failure in the treatment of infections. The aim of this study was to evaluate the prevalence of carbapenemase ...

Carbapenem resistance among Enterobacteriaceae is an emerging problem worldwide. Klebsiella pneumoniae carbapenemase (bla(KPC)) enzymes are among the most common beta-lactamases described. In this study, we report the development and validation of a real-time PCR (q-PCR) assay for the detection of bla(KPC) genes using TaqMan chemistry. The q-PCR amplification of bla(KPC) DNA was linear over 7 l...

Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; Unidade de Microbiologia e Biologia Molecular, Serviço de Patologia Clı́nica, Hospital de Clı́nicas de Porto Alegre, Porto Alegre, Brazil; Infectious Diseases Service, Hospital de Clı́nicas de Porto Alegre, Porto Alegre, Brazil; Infection Control Service, Hospital São Lucas da Pontifı́cia Universidade Católica d...

This study describes a carbapenem-resistant Klebsiella pneumoniae (CRKP) outbreak that occurred from October 2008-December 2010. Polymerase chain reaction assays were performed to detect the blaKPC gene and molecular typing was performed using pulsed-field gel electrophoresis (PFGE). There were 33 CRKP infections; PFGE revealed five genotypes: genotype A in five (15%), B in 18 (55%), C in eight...