This paper demonstrates the enrichment of reticulocytes by centrifuging whole blood through aqueous multiphase systems (AMPSs)-immiscible phases of solutions of polymers that form step-gradients in density. The interfaces of an AMPS concentrate cells; this concentration facilitates the extraction of blood enriched for reticulocytes. AMPS enrich reticulocytes from blood from both healthy and hem...

In recent years, several rapid diagnostic tests for falciparum malaria have been developed. KATTM test results were compared with microscopy on 90 consecutive patients hospitalized at the Hospital for Tropical Diseases, Bangkok, Thailand. Fifty-one patients had P. falciparum infections while 49 had malaria due to other plasmodium species. For a geometric mean ±SD (Min;Max;range) parasitemia of ...

Chitinases of trypanosomatid parasites have been proposed to fulfil various roles in their blood-feeding arthropod vectors but so far none have been directly tested using a molecular approach. We characterized the ability of Leishmania mexicana episomally transfected with LmexCht1 (the L. mexicana chitinase gene) to survive and grow within the permissive sand fly vector, Lutzomyia longipalpis. ...

BACKGROUND There are 600,000 new malaria cases daily worldwide. The gold standard for estimating the parasite burden and the corresponding severity of the disease consists in manually counting the number of parasites in blood smears through a microscope, a process that can take more than 20 minutes of an expert microscopist's time. OBJECTIVE This research tests the feasibility of a crowdsourc...

Mound-building mice, Mus spicilegus, were studied for the blood parasites in Eastern Slovakia, vicinity Kechnec village near Kosice town (Kosická kotlina basin, 21 degrees 14' E, 48 degrees 33' N) during years 2002-2005. Overall, 251 specimens were examined. The parasites were detected using microhematokrit centrifugation technique and on the Giemsa's method stained blood smears and light micro...

Anolis in the eastern Caribbean islands are hosts to three species of malaria parasite (Plasmodium). Although the parasites are widespread on the islands, little is known about their effects on infected lizards. Presented here is an inventory of some costs suffered by Anolis sabanus, the endemic solitary anole of Saba, Netherlands Antilles, when infected with Plasmodium azurophilum, Plasmodium ...

OBJECTIVE To determine the prevalence, severity and pattern of intestinal parasitic infestation in HIV-seropositive patients. METHODS A Cross-sectional study from January 2007 to December 2008.Patients were recruited from the HIV clinics of the hospital. Paired blood and single stool specimens were collected from each patient. The stool sample was investigated for intestinal parasites while t...