The precise functions of most of the proteins that participate in clathrin-mediated intracellular trafficking are unknown. We investigated two such proteins, epsinR and gadkin, using the knocksideways method, which rapidly depletes proteins from the available pool by trapping them onto mitochondria. Although epsinR is known to be an N-ethylmaleimide-sensitive factor attachment protein receptor ...

EpsinR is a clathrin-coated vesicle (CCV) enriched 70-kD protein that binds to phosphatidylinositol-4-phosphate, clathrin, and the gamma appendage domain of the adaptor protein complex 1 (AP1). In cells, its distribution overlaps with the perinuclear pool of clathrin and AP1 adaptors. Overexpression disrupts the CCV-dependent trafficking of cathepsin D from the trans-Golgi network to lysosomes ...

Multi-cycle large-eddy simulations (LES) are performed to investigate combustion cycle-to-cycle variability (CCV) in a gasoline spark ignited optical access engine operating under homogeneous stoichiometric conditions. Combustion is addressed with the Thickened Flame Model (TFM) and finite rate chemistry accounted for through reduced oxidation reaction mechanism. In view of fact that computatio...

Sir, Cases of cutaneous cysts lined by ciliated epithelium have rarely been documented, and only a few cases of such cysts in the vulvar vestibulae have been reported (1–3). The origin of the cysts is controversial because the location is so anatomically specific. In this report, we describe a 14-year-old girl with a ciliated cyst of the vulva (CCV), the lining cells of which were positive for ...

Targeting of proteins to their final destination is a prerequisite for living cells to maintain their homeostasis. Clathrin functions as a coat that forms transport carriers called clathrin-coated vesicles (CCVs) at the plasma membrane and post-Golgi compartments. In this study, we established an experimental system using Schneider S2 cells derived from the fruit fly, Drosophila melanogaster, a...

The endocytic adaptor complex AP-2 colocalizes with the majority of clathrin-positive spots at the cell surface. However, we previously observed that AP-2 is excluded from internalizing clathrin-coated vesicles (CCVs). The present studies quantitatively demonstrate that AP-2 disengages from sites of endocytosis seconds before internalization of the nascent CCV. In contrast, epsin, an alternate ...

Spotted gum (Corymbia citriodora spp. variegata; CCV) has been widely planted, a wide natural distribution, and is the most important commercially harvested hardwood species in Queensland, Australia. It great capacity to sequester carbon, thus reducing impact of CO2 emissions on climate. Belowground root biomass (BGB) plays an role as carbon sink terrestrial ecosystems. To explore potential acc...

Comentario legislativo del Decreto número 120 que reforma y adiciona diversas disposiciones de la Ley Acceso las Mujeres a una Vida Libre Violencia para el Estado Sonora, Instituciones Procedimientos Electorales Orgánica Fiscalía General Justicia Estatal Responsabilidades, Poder Ejecutivo Judicial De Legislativo Sonora Gobierno Administración Municipal. Boletín Oficia t. CCV, Edición Especial, ...