In this paper, the position of crossing linkage between cell wall polysaccharides was studied by determining the digestibility of pectin-depleted walls, the contents of hydrocinamic acids and their dimmers in each fraction of elongated and non-elongated cells. The results showed that Ox. sol. fr. was composed mainly of pectin polysaccharides while 4K sol. fr. was composed both of pectin and hem...

Polysaccharide-rich plant cell walls are hydrated under functional conditions, but the molecular interactions between water and polysaccharides in the wall have not been investigated. In this work, we employ polarization transfer solid-state NMR techniques to study the hydration of primary-wall polysaccharides of the model plant, Arabidopsis thaliana. By transferring water (1)H polarization to ...

BACKGROUND Plant biomass degradation by fungal-derived enzymes is rapidly expanding in economic importance as a clean and efficient source for biofuels. The ability to rationally engineer filamentous fungi would facilitate biotechnological applications for degradation of plant cell wall polysaccharides. However, incomplete knowledge of biomolecular networks responsible for plant cell wall decon...

Cell walls are metabolically active components of plant cells. They contain diverse enzymes, including transglycanases (endotransglycosylases), enzymes that 'cut and paste' certain structural polysaccharide molecules and thus potentially remodel the wall during growth and development. Known transglycanase activities modify several cell-wall polysaccharides (xyloglucan, mannans, mixed-linkage β-...

The aim of this work was to investigate heat-induced changes in cell wall polysaccharides of carrot in relation to texture. Discs of fresh carrot (Daucus carota cv Amstrong) tissue were subjected to cooking (100¡C, 20 min), with or without a pre-cooking treatment (50¡C, 30 min). Alcohol-insoluble residues were prepared from the tissues and were extracted sequentially with water, NaCl, CDTA, and...

Grasses represent an abundant and widespread source of lignocellulosic biomass, which has yet to fulfil its potential as a feedstock for biorefining into renewable and sustainable biofuels and commodity chemicals. The inherent recalcitrance of lignocellulosic materials to deconstruction is the most crucial limitation for the commercial viability and economic feasibility of biomass biorefining. ...

Studies of the primary structures of polysaccharides of growing plant cell walls have shown that these structures are far more complex than was anticipated just a few years ago. This complexity can best be appreciated by considering xyloglucan, a hemicellulose present in the cell wall of both monocots and dicots, and rhamnogalacturonan II (RG-II) and rhamnogalacturonan I (RG-I), two structurall...

Cell wall storage polysaccharides (CWSPs) are found as the principal storage compounds in seeds of many taxonomically important groups of plants. These groups developed extremely efficient biochemical mechanisms to disassemble cell walls and use the products of hydrolysis for growth. To accumulate these storage polymers, developing seeds also contain relatively high activities of noncellulosic ...

The physical properties exerted by dietary fibre are individual to the age and aiiatomy of the fruit or vegetable eaten. The physical properties will be determined by the chemistry of the constituent polysaccharides and lignins, In the plant these rnacromolecules arc systematically intermeshed for anatomical and physiological function. This intimate admixture is lost during digestion but the ma...

“At the beginning of the 1960s, it was impossible to quantitate accurately the neutral sugar compositions of complex carbohydrates. A major step towards such quantitation was achieved by Charles Sweeley and his colleagues who showed that volatile derivatives of sugars could be used for quantitative analysis by gas chromatography.1 The Sweeley group synthesized trimethylsilyl derivatives. These ...