objective: diacylglycerol acyltransferase 1 is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. the objective of this project is to check out the polymorphism at the exon 8 region of dgat1 gene using pcr-sscp technique in iranian water buffaloes (bubalus bubalis). recent activities have shown that a significant association between lysine at amino acid position 232 wi...

In this study we estimated the allele and genotype frequencies of SCD1 and DGAT1 gene polymorphism. The analysis was conducted on 408 Holstein cows from five dairy herds in Isfahan province. Genotypes were identified using PCR-RFLP technique. Two genetic variants (A and V) of the SCD1 gene were observed in this experiment. The frequency of A-allele ranged from 0.49 to 0.66, while frequency of V...

Acyltransferases are key contributors to triacylglycerol (TAG) synthesis and, thus, are of great importance for seed oil quality. The effects of increased or decreased expression of ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) or PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE (PDAT) on seed lipid composition were assessed in several Camelina sativa lines. Furthermore, in vitro assays of...

KADLECOVA, V., D. NEMECKOVA, K. JECMINKOVA and L. STADNIK, 2014. The effects of polymorphism in the DgAt1 gene on energy balance and milk production traits in primiparous Holstein cows during the first six months of lactation. Bulg. J. Agric. Sci., 20: 203-209 The aim of this study was to analyze the effects and significance of DGAT1 K232A polymorphism on milk production traits – milk yield, co...

BLAT (BLAST-Like Alignment Tool) analyses of the opossum (Monodelphis domestica) and zebrafish (Danio rerio) genomes were undertaken using amino acid sequences of the acylglycerol acyltransferase (AGAT) superfamily. Evidence is reported for 8 opossum monoacylglycerol acyltransferase-like (MGAT) (E.C. 2.3.1.22) and diacylglycerol acyltransferase-like (DGAT) (E.C. 2.3.1.20) genes and proteins, in...

Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide. NAFLD usually begins as low-grade hepatic steatosis which further progresses in an age-dependent manner to nonalcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and hepatocellular carcinoma in some patients. Ghrelin is a hormone known to promote adiposity in rodents and humans, but its potential role in hep...