Although in most cases of haemolytic disease of the newborn practically all the bilirubin in the serum is of the indirect reacting type, a quarter of a century ago Still (1927) and Hawksley and Lightwood (1934) described cases in which the serum direct reacting bilirubin was raised and signs of obstructive jaundice appeared. This condition came to be known as the 'inspissated bile syndrome' (Hs...

A small regional survey of direct spectrophotometric methods and a larger Australian and New Zealand survey of paediatric bilirubin analyses are described. The overall performance of both groups was unsatisfactory with an unacceptable high interlaboratory variation. This interlaboratory variation was reduced significantly by the use of a spectrophotometric method with a common standard of methy...

OBJECTIVE To test two water soluble extracts (aqueous and ethanolic) obtained from the leaves of Vitex doniana in normal and streptozotocin-induced diabetic rats for their effects on pancreatic endocrine tissues and serum marker enzymes for a period of 21 d. METHODS A total of 55 rats divided into 11 groups of 5 rats each were assigned into diabetic and non-diabetic groups and followed by a d...

The aims of this study were to determine the incidence of UTI in asymptomatic jaundiced neonates in the first two weeks of life , and to determine which historical and laboratory parameters are associated with UTI in those neonates.The study included 153 full -term jaundiced neonates less than two weeks old who had been admitted in hospital for management of jaundice. Work-up for hyperbilirubin...

introduction: neonatal hyperbilirubinemia is one of the most important factors affecting the auditory system and can cause sensorineural hearing loss. this study investigated the relationship between behavioral hearing thresholds in children with a history of jaundice and the maximum level of bilirubin concentration in the blood.   materials and methods: this study was performed on 18 children ...

The form in which bilirubin exists in the blood is of considerable interest because of the observed differences in the van den Bergh reaction in disease and because of the relation of bilirubin to hemoglobin metabolism. All of the bilirubin in plasma reacts in 30 minutes with diazonium salts in acid 50 per cent methyl alcohol to give a red dye (1). A variable fraction of the total reacts with v...

A high (2.0 mol/L) NaCl concentration apparently suppresses the secondary-site binding of bilirubin by human serum albumin. Thus if an excess of bilirubin is added to human serum albumin or to neonatal serum in buffer containing 0.1 mol of tris(hydroxymethyl)aminomethane and 2.0 mol of NaCl per liter (pH 7.5), and the bilirubin not bound to albumin is removed by treatment with calcium carbonate...

THE pigments present in the blood-serum in jaundice were first differentiated by van den Bergh, who found that, whereas all icteric sera coupled with diazobenzene-p-sulphonic acid (Ehrlich's diazo-reagent) in the presence of alcohol, only certain of these sera would couple in simple aqueous solution. These diazo-reactions van den Bergh termed "indirect" and "direct" respectively, and it is now ...

OBJECTIVE To determine the usefulness of the hour-specific Bhutani et al bilirubin nomogram when applied to infants with Coombs-positive test results. DESIGN Retrospective chart review. SETTING Term nursery and neonatal intensive care unit of a university-affiliated hospital. PATIENTS All infants with A+ or B+ blood type born in our center from September 1, 2006, through August 31, 2008, ...