Traditionally, marrow donor registries and transplant centers collect DNA samples from potential donors through either a blood sample or a buccal swab sample. Both of these sample types present some challenges as blood collections are invasive for the donor and swabs present time and sample quality issues. These challenges can be eliminated by using ORAcollect®•DNA to collect a DNA sample from ...

Material and Methods: This study included 307 subjects (250 healthy individuals and 57 patients with ankylosing spondylitis who were diagnosed at the University Clinic of Rheumatology in Skopje). The HLA typing of class 1 (HLA-A, HLA-C and HLA-B) genes was performed using the method of Reverse Line Strip, after isolation of DNK from the blood leucocytes with the standard phenol-chloroforme meth...

Objective HLA-A*11:256Q and HLA-A*02:621 are two low-frequency HLA-A alleles. The aim here is to report the ethnicity of A*11:256Q and A*02:621 and associated human leukocyte antigen (HLA) haplotypes among Taiwanese individuals. Materials and Methods HLA data from randomized Taiwanese registered in the Tzu Chi Stem Cells Centre and China Shanghai Tissuebank Diagnostics were analyzed. HLA typi...

background: the objective of this study was to investigate hla-drb1*and dqb1* allelic polymorphisms in iranian patients with hydatidose. this is the first survey dealing with the correlation between hla-drb1* and dqb1* al-leles and cystic echinococcosis in iranian patients. methods : the study was carried out on 56 patients with confirmed cystic echi-nococcosis and 30 apparently healthy individ...

Although extensive HLA-A and HLA-B polymorphism is evident, the true diversity of HLA-C has remained hidden due to poor resolution of HLA-C Ags. To better understand the polymorphic nature of HLA-C molecules, 1823 samples from the National Marrow Donor Program research repository in North America have been typed by DNA sequencing and interpreted in terms of HLA-C diversification. Results show t...

It is widely accepted that donor leukocytes survive within the recipient periphery after blood transfusion or solid organ transplantation. The significance of this microchimerism remains unclear, partially because of the insecurity of assays used to detect the donor-derived material. The techniques used to detect donor-derived DNA within recipient peripheral blood rely largely on major histocom...

To investigate the human leukocyte antigen (HLA) association with anti-synthetase syndrome (ASSD). We conducted largest immunogenetic HLA-DRB1 and HLA-B study to date in a homogeneous cohort of 168 Caucasian patients ASSD 486 ethnically matched healthy controls by sequencing-based-typing. A statistically significant increase HLA-DRB1*03:01 HLA-B*08:01 alleles compared was disclosed (26.2% versu...

Background Human leukocyte antigen (HLA) gene is a highly polymorphic region. HLA typing is required to match patients and donors for transplantation; therefore, development of HLA registries is necessary for finding HLA match donors. HLA system is highly informative, and numerous studies have been conducted on HLA allele distribution in different populations. Methods In this study, 100 unrel...

The frequencies of HLA-antigens in A, B, C and DR loci were examined in 34 Japanese patients with acromegaly. The frequency of DRW9 was slightly higher and the frequency of DR4 was slightly, but not significantly, lower than the controls. Five HLA loci (A, B, C and D/DR loci) have been found and other new loci are expected to be disclosed. Since the associations of HLA with diseases began being...