serology and dna techniques are employed for human leukocyte antigen (hla) typing in different transplant centers. results may not always correlate well and may need retyping with different technique. all the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring hla typing for bone marrow transplant were enrolled in the study. serological hla typing was ...

the last two decades have seen a massive growth in the application of dna technology in histocompatibility and immunogenetics. this review summarises the history and application of dna methods in this area.

background: the human leukocyte antigen-drb1 (hla-drb1) locus is one of the most polymorphic human loci and has a crucial role in the immune system. assessing the allelic frequencies of hla-drb1 locus would be a fundamental factor in defining the origin of populations, relationships with other populations, disease association studies and the constitution of unrelated bone marrow donor registrie...

Serology and DNA techniques are employed for Human Leukocyte Antigen (HLA) typing in different transplant centers. Results may not always correlate well and may need retyping with different technique. All the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring HLA typing for bone marrow transplant were enrolled in the study. Serological HLA typing was ...

introduction: considering the role of hla matching in transplant outcome, the quality of hla-dr typing is clearly an important issue.in recent years serologic methods have been replaced with dna based typing methods.the main objective of this study was to compare hla-dr typing data obtained from existing serologic method with that obtained by the new pcr-ssp method. material and methods: fifty-...

DNA based HLA typing is fast replacing conventional Microlymphocytotoxicity based method, which has been regarded as the gold standard. Many laboratories in Mumbai have already switched over to molecular methods, as the results are far superior. This study was undertaken to compare the results of generic molecular typing by sequence specific primers (SSP) with that by serology in our laboratory...

Objective : a retrospective analysis of the results obtained by typing HLA-A, -B antigens and HLA DRB1 allele groups in patients with rheumatoid arthritis (RA) Rostov population. Materials methods rheumatic inflammatory diseases who were on inpatient treatment rheumatology department SBI “OKB No. 2” was carried out LITT RO "BTS" 2019-2020. 41 (9 men, 32 women, median age — 42 years) diagnosed R...

Unrelated stem cell registries have been collecting HLA typing of volunteer bone marrow donors for over 25years. Donor selection for hematopoietic stem cell transplantation is based primarily on matching the alleles of donors and patients at five polymorphic HLA loci. As HLA typing technologies have continually advanced since the beginnings of stem cell transplantation, registries have accrued ...

In comparison with HLA-matched sibling bone marrow transplants, unrelated donor transplants are associated with increased graft-versus-host disease and graft failure. This is likely in part due to HLA incompatibilities not identified by current matching strategies. High resolution DNA-based typing methods for HLA class II loci have improved donor selection and treatment outcome in unrelated don...