نتایج جستجو برای: enzyme electrodes
تعداد نتایج: 279932 فیلتر نتایج به سال:
Nanostructured ZnO with its unique properties could provide a suitable microenvironment for immobilization of enzymes while retaining their biological activity, and thus lead to an expanded use of this nanomaterial for the construction of electrochemical biosensors with enhanced analytical performance. ZnO-based enzyme electrochemical biosensors are summarized in several tables for an easy over...
This paper reports a simple DNA sensor having a detection limit of about 24 oligonucleotides and that operates without the need for PCR amplification. The sensor platform is based on an interdigitated array (IDA) of electrodes. The electrodes are modified with DNA capture probes, which are complementary to an analog for the Epstein-Barr genome, and then exposed to an alkaline phosphatase-labele...
Cholinesterase sensors based on screen-printed electrodes modified with polyaniline, 7,7',8,8'-tetracyanoquinodimethane (TCNQ), and Prussian blue have been developed and tested for detection of anticholinesterase pesticides in aqueous solution and in spiked grape juice. The influence of enzyme source and detection mode on biosensor performance was explored. It was shown that modification of the...
The entrapment method was used to prepare electrochemical biosensors. Using controlled potential method, copolymers were synthesized between aniline y sulfonated-anilines in aqueous media at pH 4.9 on Platinum disc. The copolymers were characterized by elemental analysis and spectroscopy. Glucose-oxidase was entrapped in the electropolymerization process, at pH 4.9 in aqueous media. The copolym...
Au nanocrystals with different morphologies were prepared and used for enzyme-free electrochemical biosensor applications. To investigate the electrocatalytic properties of Au nanocrystals as a function on their morphologies, Au nanocrystals, Au nanospheres (NSs) on silica, Au NSs, and Au nanorods (NRs) with aspect ratios of 1:3 and 1:5, were coated on the screen printed electrodes and further ...
The effects of ionic liquids (ILs) on the catalytic activity of enzymes were studied by approaches of electrochemistry and quantum chemistry calculation in this work. Three types of ILs, namely, [bmpyri]BF(4), [bmpyrro]BF(4), and [bmim]BF(4), were selected to address the effects of different types of ILs on the electrocatalytic activity of glucose oxidase (GOx) toward the oxidation of glucose. ...
A novel method for preparing enzyme membranes was developed. The enzyme was attached onto the electrode surface by dropping the enzyme solution and allowing it to dry. Glucose oxidase was used for entrapment. Then, the electrode surface was coated with an ionic liquid containing cellulose, and the ionic liquid was removed by immersing the electrode into water. Enzyme activity was retained in th...
Glucose sensing electrodes have been realized by immobilizing glucose oxidase (GOx) on unmodified edge plane of highly oriented pyrolytic graphite (epHOPG) and the native oxide of heavily doped silicon (SiO2/Si). Both kinds of electrode show direct interfacial electron transfer due to the redox process of the immobilized GOx. The measured formal potential of the redox process agrees with that o...
The present paper deals with the design of experimental work matrices for two groups of experiments by using Response surface methodology (RSM). The first EDM group was dealt with the use of kerosene dielectric alone, while the second was treated by adding the graphite micro powders mixing to dielectric fluid (PMEDM). The total heat flux generated and fatigue lives after EDM and PMEDM models we...
The first enzyme-based catalyst that is superior to platinum in the four-electron electroreduction of oxygen to water is reported. The smooth Pt cathode reached half and 90% of the mass transport-limited current density at respective overpotentials of -0.4 and -0.58 V in 0.5 M sulfuric acid, and only at even higher overpotentials in pH 7.2 phosphate buffer. In contrast, the smooth "wired" bilir...
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