objective: the present study was an attempt to study total phenolic content and antioxidant property of the crude ethanolic extract of the roots of azadirachta indica (a. indica). materials and methods: to evaluate the antioxidant properties of the crude extract, some complementary test systems, namely dpph free radical scavenging assay, reducing power assay, and ferrous ion chelating ability a...

abstractbackground : in order to quantify effects of ultrasound irradiation parameters under therapeutic condition, especially sonodynamic therapy, it is initially necessary to evaluate inertial cavitation activity in vitro conditions therefore, in this study, the effect of 1 mhz low level ultrasound based on °oh radicals generated by acoustic inertial cavitation in aqueous solution was monitor...

Simply aqueous sodium hydroxide is sufficient to exclude ionic side reactions and to prepare 2-aminobiphenyls from aryl diazotates and anilines through a new variant of the Gomberg-Bachmann reaction (see scheme). The metal-free reaction under basic conditions allows to exploit the highly radical-stabilizing effect of the aniline's free amino function for the first time, which leads to a so far ...

a vast majority of the studies addressing the free radicals including hydroxyl radical is a damage compound of biochemical molecules such as dna, proteins and lipids. when free radicals specially hydroxyl radical are not adequately removed from the body, it may damage biological macromolecules, leading to a variety of disease occurs. therefore, the body should be protected by an enzymatic or no...

Reaction energetics of the double (2H+/2e-), i.e., the first 1H+/1e- (catechol→ phenoxyl radical) and the second 1H+/1e- (phenoxyl radical→ quinone) free radical scavenging mechanisms of quercetin and its six colonic catecholic metabolites (caffeic acid, hydrocaffeic acid, homoprotocatechuic acid, protocatechuic acid, 4-methylcatechol, and catechol) were computationally studied using density fu...

α-Carboxylate radical anions are potential reactive intermediates in the free radical oxidation of biological molecules (e.g., fatty acids, peptides and proteins). We have synthesised well-defined α-carboxylate radical anions in the gas phase by UV laser photolysis of halogenated precursors in an ion-trap mass spectrometer. Reactions of isolated acetate (˙CH(2)CO(2)(-)) and 1-carboxylatobutyl (...

Kinetics of enzymic oxidation of indoleacetic acid (ISA) are interpreted as indicating that the reaction has an autocatalytic, cyclical mechanism invol\-ing unst,ablc intermcdiatcs whose formation and disappearance are important in determining the over-all reaction rate. The kinetics do not supporl the idea that IAA oxidation occurs mainly by reaction with Mnt3, nor that the reaction is an elec...

Background & Aim: Medicinal plants can be a good replacement for common cancer treatment including chemotherapy, radiation therapy and surgery because they don’t have many side effects. The aim of this study was to investigate the effect of temperature on the free radical scavenging in the presence of medicinal plants. Experimental: For determination of free radical scavenging activity was use...

Brain tissue being rich in polyunsaturated fatty acids, is very susceptible to lipid peroxidation. Iron is well known to be an important initiator of free radical oxidations. We propose that the principal route to iron-mediated lipid peroxidations is via iron-oxygen complexes rather than the reaction of iron with hydrogen peroxide, the Fenton reaction. To test this hypothesis, we enriched leuke...

Free radicals are thought to be key intermediates in the oxidation of wine, but their nature has not been established. Electron paramagnetic resonance spectroscopy was used to detect and identify several free radical species in wine under oxidative conditions with the aid of spin traps. The 1-hydroxylethyl radical was the sole radical species observed when α-(4-pyridyl-1-oxide)-N-tert-butylnitr...