This study is pertaining to the production of penicillin G acylase (PGA) by Bacillus subtilis 8105MU331 in which PGA gene, under the control of thermal-induced promoter, was integrated. The key process parameters including induced-temperature, induced-time, and culture temperature were optimized in flask culture. A three-stage cultivation process was developed for PGA production with the expres...

to assess the effect of non chemical management of weed control on forage sorghum (sorghum bicolor) a field study was conducted in varamin, iran during 2010 crop year in a three- replicated- split factorial experiment laid out in randomized complete block design with four weeding levels (w1= one time cultivation at 3-leaf stage using a duck foot cultivator, w2= two times cultivation at 3 and 5-...

This study investigated the cultivation of the marine microalga Chlorella sp. without supplying an inorganic carbon source, but instead with enriching the media with extracts of oyster shells pretreated by a high-pressure homogenization process. The pretreated oyster shells were extracted by a weak acid, acetic acid, that typically has harmful effects on cell growth and also poses environmental...

the effect of 10-year continuous saffron cultivation on physical and chemical properties of a silty clay loam soil was investigated in a research farm of college of agriculture, shiraz university, shiraz (52 º 33ʹ e longitude and 29º 36ʹ n latitude), iran. this farm was irrigated with different regimes (100% of saffron's potential evapotranspiration (etp), 75%etp, 50%etp) including rain fed tre...

Methanol limited fed-batch cultivation was applied for production of a plant derived beta-glucosidase by Pichia pastoris. The beta-glucosidase was recovered by expanded bed adsorption chromatography applied to the whole culture broth. The new Streamline Direct HST1 adsorbent was compared with Streamline SP. Higher bead density made it possible to operate at two times higher feedstock concentrat...

BACKGROUND For their application in the area of diagnosis and therapy, single-domain antibodies (sdAbs) offer multiple advantages over conventional antibodies and fragments thereof in terms of size, stability, solubility, immunogenicity, production costs as well as tumor uptake and blood clearance. Thus, sdAbs have been identified as valuable next-generation targeting moieties for molecular ima...

background: in megavoltage radiotherapy rooms, ordinary concrete is usually used due to its low construction costs, although higher density concrete are sometimes used, as well. the use of high-density concrete decreases the required thickness of the concrete barrier hence, its disadvantage is its high cost. in a nuclear reactor, neutron radiation is the most difficult to shield. a method for p...