The Rhizopus oryzae species complex is a group of zygomycete fungi that are common, cosmopolitan saprotrophs. Some strains are used beneficially for production of Asian fermented foods but they can also act as opportunistic human pathogens. Although R. oryzae reportedly has a heterothallic (+/-) mating system, most strains have not been observed to undergo sexual reproduction and the genetic st...

The degradation rate of 3-hydroxy-3-methylglutaryl CoA reductase (HMG-R), a key enzyme of the mevalonate pathway, is regulated through a feedback mechanism by the mevalonate pathway. To discover the intrinsic determinants involved in the regulated degradation of the yeast HMG-R isozyme Hmg2p, we replaced small regions of the Hmg2p transmembrane domain with the corresponding regions from the oth...

The biosynthesis of isopentenyl diphosphate (isopentenyl pyrophosphate), the precursor of isoprenoids in all forms of life, occurs by two distinct metabolic pathways, the mevalonate pathway (Fig. 1) and the glyceraldehyde 3-phosphate/pyruvate pathway, often termed the nonmevalonate pathway (17). Whereas many gram-negative bacteria employ the nonmevalonate pathway (26), humans, other eukaryotes,...

Progression of colon cancer is associated with the up-regulation of cyclooxygenase-2 (COX-2) and hydroxymethyl glutaryl CoA reductase (HMG-R). Clinical and preclinical evidence shows that a combination of COX-2 and HMG-R inhibitors provide additive/synergistic chemopreventive effects against colorectal cancer. However, the mechanism by which statins and NSAIDs inhibit cancer growth is not yet f...

The high mobility group (HMG) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (HPLC). Reversed-phase HPLC under conditions of 50 mM triethylamine adjusted to pH 2.2 with phosphoric acid (solvent A) and 95% acetonitrile in water (solvent B) was used to separate proteins primarily on the basis of differences in the overall hydrophobi...

Altered cholesterol homeostasis has been noted in malignant cells, which led us to explore the regulation of cholesterol metabolism in normal and leukemic cells. The mean low-density lipoprotein (LDL) receptor and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activities were fivefold and threefold higher in mononuclear blood cells from 33 patients with leukemia, compared with cells ...

خلا صلا ـ ة : عون نم يفيكتلا ةانقلا ردقم ءادلأ اًضرع ثحبلا اذه مدقي LMS ةيئاضفلا ةرفشملا تاعومجملا مظن يف نييئاوه مدختست يتلا ةينمزلا لإل اسر ل لابقتسلال اًدحاو اًيئاوهو . و ةانق يه ةمدختسملا ةيكلسلالا ةانقلا Rayleigh ءاضوضلا دوجوب ىسواجلا (Gaussian) يمجتلا جارخلاا يف يع . و حضوت ثحبلا جئاتن إ دحاو لماعم يذ ةانق ردقم مادختساب زاحنم ريغ ريدقت ىلع لوصحلا ةيناكم ) يراسم نم لكل لإا ةراش .( و وحن ةيف...

In this work we study the effect of some electrical prosperities on synthetic rubber reinforced with carbon black diluted in heptanes with concentrations from 0.2% gm/mole to 2.0% gm/ mole in temperatures range (100, 150, 200, 250, 300) C associated with amount weight of black carbon 5wt% added to the diluted rubber. The results of electrical properties measurement of Buna rubber reinforced wit...

The Smith-Lemli-Opitz syndrome (SLOS) is a common birth defect-mental retardation syndrome caused by a defect in the enzyme that reduces 7-dehydrocholesterol to cholesterol. Because of this block, patients' plasma cholesterol levels are generally low while 7-dehydrocholesterol concentrations are markedly elevated. In addition, plasma total sterols are abnormally low and correlate negatively wit...