OBJECTIVE To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development. DESIGN Animal study. SETTING University laboratory. ANIMAL(S) CD-1 mice. INTERVENTION(S) Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 micromol/L, 5 micromol/L, and 10 mic...

Sunflower is an important oilseed crop, however the genetic transformation of sunflower has been very challenging to date. In the present study, we describe a successful Agrobacterium-mediated transformation approach via somatic embryogenesis from immature zygotic embryos of sunflower. By optimizing the culture medium, we have achieved high efficiency (45.5%) of somatic embryogenesis with MS me...

Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. ...

This report describes a live birth produced from repeat vitrification and thawing of blastocysts derived from in-vitro matured (IVM) oocytes in a woman with polycystic ovarian syndrome. Immature oocyte retrieval was performed on day 12 of her induced menstrual cycle. The patient was administered 10,000 IU of human chorionic gonadotrophin s. c. 36 h before immature oocyte retrieval. A total of 4...

Embryo transfer refers to a step in the process of assisted reproduction in which one or several embryos are placed into the uterus of a female with the intent to establish a pregnancy. Currently this biotechnology has become one of the prominent high businesses worldwide. This technique, which is often used in connection with in vitro fertilization (IVF), has widely been used in animals or hum...

Abstract Compared to somatic or sperm cells, mammalian oocytes are much more sensitive cryopreservation mainly because of the large volume, cytoskeleton, and presence zona pellucida. Connections with surrounding cumulus cells also challenging preserve in immature oocytes. Besides genetic epigenetic information enclosed nucleus, contain organelles cytoplasmic factors that necessary for early emb...