Immunofluorescent staining techniques were used to study the distribution of the Ca(2) + Mg(2+)-dependent ATPase and calsequestrin in primary cultures of differentiating rat skeletal muscle cells, grown for different periods of time under various culture conditions. In mononucleated myoblasts calsequestrin was detected after 45 h in culture whereas the ATPase was not detected until 60 h. After ...

Immunofluorescent localization of aquaporin 5 (AQP5) was investigated in rat ovarian follicles during development and preovulatory cumulus oophorus expansion. Ampullary cumuli oophori complexes (COCs) were examined. Analysis revealed that AQP5 immunostaining appeared in preantral follicles and formed a characteristic ring encircling and touching the oolemma. The staining represented most likely...

introduction antineutrophil cytoplasmic antibodies (anca)-associated glomerulonephritis (gn) is characterized by necrotizing and crescentic gn with paucity of immunoglobulin (ig) and complement deposition, which is also known as pauci-immune crescentic gn. membranous nephropathy (mn) is characterized by the formation of subepithelial immune deposit with resultant changes in glomerular basement ...

Current skin substitutes fail to meet the need for replacement in full-thickness wounds. Bioprinting is a promising alternative method generate substitutes, as it can replicate structural organization of into biomimetic layers. In prior studies, we demonstrated that wounds treated with bioprinted had accelerated wound healing driven by epithelialization reduced contraction and immunofluorescent...

There are many effective chemothereutic agents used in influenza disease which some of them inhibit virus replication by interfering with FluV (influenza virus) viral binding or its penetration into cell membrane. A series of polyoxometalates compounds such as POM-523 and PM-504 have been synthesized and have showed inhibitory effects on viruses. In this study we examined anti influenza activit...

PURPOSE To investigate the distribution of inflammatory mediators such as interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha and angiogenic cytokines such as vascular endothelial growth factor (VEGF) and to identify their cellular source in surgically excised choroidal neovascular membranes (CNVMs) of various origins. METHODS Immunoperoxidase staining was performed on paraffin-embed...

Jones, Wallis L. (Communicable Disease Center, Atlanta, Ga.), and Vester J. Lewis. Role of bacterial chemical components in immunofluorescence. J. Bacteriol. 91:1700-1704. 1966.-The problem of heterologous staining of bacteria in pure culture and in clinical specimens by fluorescein-labeled antisera for Corynebacterium diphtheriae was examined by observing the presence of particular cellular co...

Immunofluorescent staining of tissue from a lung tumor detected 12 years after excision of a primary malignant melanoma of the skin was negative for prekeratin and positive for vimentin, indicating that the tumor was not epithelial in origin and excluding carcinoma from the differential diagnosis. Complementary conventional staining with hematoxylin-eosin confirmed the melanocytic origin of the...

Stevens Johnson syndrome (SJS)/ toxic epidermal necrolysis (TEN) are characterized by the remarkable cell death of keratinocytes. Our previous studies have revealed that keratinocyte pathway involve apoptosis and necroptosis. However, in recent years, it has been found there is a crosstalk between apoptosis, necroptosis pyroptosis. Pyroptosis an inflammatory programmed caspase 1-mediated cleava...

The distribution of DNA topoisomerase I within Drosophila polytene chromosomes was observed by immunofluorescent staining with affinity-purified antibodies. The enzyme is preferentially associated with active loci, as shown by prominent staining of puffs. The heat shock loci 87A-87C are stained after, but not before, heat shock induction. A detailed comparison of the distribution of topoisomera...