The present study has evaluated the association of growth differentiation factor9 (GDF9) and bone morphogenetic protein15 (BMP15) mRNA expression in cumulus-oocyte complexes (COCs) of buffalo ovary during in vitro maturation (IVM). GDF9 and BMP15 are expressed specifically in mammalian oocytes and also participate in cumulus-oocyte crosstalk. Quantitative real-time polymerase chain reaction (qR...

background: ovarian tissue transplantation is emerging technologies for fertility preservation. in addition, in vitro maturation (ivm) of oocytes retrieved from ovarian tissues may overcome the fertility defects in certain cases. objective: the aim was to evaluate the best site for ovarian tissue transplantation in mice. also, feasibility of ivm of oocytes retrieved from auto grafted ovarian ti...

a:4:{s:10:"Background";s:513:"Therapeutic potential of in vitro maturation (IVM) in infertility is growing with great promise. Although significant progress is obtained in recent years, existing IVM protocols are far from favorable results. The first aim of this study was to investigate whether two step IVM manner change reactive oxygen species (ROS) and total antioxidant capacity (TAC) levels....

Background: Setting up an in vitro follicle culture system that resembles in vivo ovary condition has high value in research. Additionally, expression evaluation of folliculogenesis involved genes could lead us to the designing of better culture system. Materials and Methods: ovaries of 12-day-old female NMRI mice were removed, 100-130 μm pre-antral follicles were mechanically isolated from fre...

This study examined the effects of human menopausal gonadotrophin (HMG) or human chorionic gonadotrophin (HCG) priming on cumulus-oocyte complex (COC) morphology, oocyte maturation and embryo development in patients undergoing in-vitro maturation (IVM) cycles. The patients were primed with nothing (group 1), low-dose HMG (group 2) or 10,000 IU HCG (group 3) before oocyte retrieval. COC with dis...

Background: The effects of cumulus cells and the effectiveness of a PDE3 specific inhibitor (Cilostamide) in comparison with an adenylate cyclase activator (Forskolin) on the maturation rate and developmental competence of mouse germinal vesicle (GV) oocytes were evaluated Materials and Methods: Germinal vesicles (GV) oocytes of PMSG-primed mice were divided into cumulus denuded oocytes (CDOs) ...

We analyzed the expression of mRNAs for growth factor [epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), basic fibroblast growth factor (bFGF) and platelet-derived growth factor A chain (PDGF-A)] and their receptor (R) by reverse transcription-polymerase chain reaction in bovine ova during oocyte maturation in vitro (0-21 hr) and after fertilization in vitro (6-144 hr: zygote...

BACKGROUND It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger. OBJECTIVE Effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. MATERIALS AND METHODS Oocytes from supper-ovulated mouse were divided to two groups: cumulus...

BACKGROUND MicroRNA (miRNA) are small non-coding RNA molecules critical for regulating cellular function, and are abundant in the maturing oocyte and developing embryo. MiRNA-21 (MIR21) has been shown to elicit posttranscriptional gene regulation in several tissues associated with rapid cell proliferation in addition to demonstrating anti-apoptotic features through interactions with PDCD4 mRNA ...

BACKGROUND Leptin, as a 16 kDa adipokine, is a pleiotropic cytokine-like hormone that primarily secreted from adipose tissue. It also involves in the regulation of energy homeostasis, neuroendocrine function, immunity, lipid and glucose homeostasis, fatty acid oxidation, angiogenesis, puberty and reproduction. The aim of this study was to investigate the effects of in vitro addition of leptin t...