background: as we all know, sperm has the capacity to take up foreign dna, therefore, sperm mediated gen transfer can be an inexpensive and simple method in animal transgenesis in various species. however, there is not sufficient evidence of dna uptake by ovine spermatozoa. objectives: the purpose of the present study was to examine the uptake of human lysozyme gene contained plasmid (pegfp-ire...

Introduction: In chick embryo, endodermal component of the liver becomes split into 2 diverticula in a short time. The anterior diverticulum lies to the left of the ductus venosus and the posterior one to its right side. As the liver primordium grows, it is invaded by the embryonic veins. Based on the different incubation time periods and evolutionary origin of avian species...

1. A direct fluorescent antibody (F.A.) tech­nique for diagnosis of Gonococci is de­scribed. 2. The results of applying F.A. method in the 270 female cases are presented and compared with the conventional method find­ings. 3. 220 cases were diagnosed by one or more methods at the first examinations. F .A. tests alone were positive in 44 ,eases and .F .A. direct diagnosis was made in a tot...

This study was carried out to examine aflatoxin B1 (AFB1) removal ability of four strains of lactic acidbacteria (LAB). Three indigenous (Lactobacillus rhamnosus TMU094, Lactobacillus fermentum TMU121and Pedioccus pentosaceus TMU457) and a non-indigenous (Labacillus rhamnosus PTCC1637) isolateswere studied. The strains were incubated with (AFB1) at different time. The toxin residual in the supe...

OBJECTIVE To analyze the meanings produced in the Health Surveillance actions for tuberculosis control, carried out by healthcare professionals in Mozambique. METHOD Qualitative study using the theoretical and methodological framework of the French Discourse Analysis. RESULTS A total of 15 healthcare professionals with more than one year of experience in disease control actions participated...

This paper describes optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of L-asparaginase by Serratia marcescens SB08. Four medium factors, from out of 11 medium factors, were screened by Plackett-Burman design experiments and subsequent optimization process to find out the o...

We have investigated the effect of theophylline on the kinetics of the catalytic subunit of protein kinase and related factors in lung tissue. The results show that the point of highest concentration of the C subunit of protein kinase which is active in casein phosphorylation is at 3h of incubation time, but in the presence of 100 Ilg/ InL and 10µg/mL theophylline, this is shifted to I.S an...

Background: Detection of sperm parameters based on conventional methods, immediately after thawing, have been revealed sperm capacity to in vivo and in vitro fertilization. But this evaluation is not fully reliable because of long journey of sperm during female genital tract to reach the oocyte and fertilize it. Therefore, analyzing postthawed sperm parameters after incubation is more beneficia...

this paper describes optimization method that combines the plackett-burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of l-asparaginase by serratia marcescens sb08. four medium factors, from out of 11 medium factors, were screened by plackett-burman design experiments and subsequent optimization process to find out the o...