A total of 61 clinical yeast isolates of Candida, Cryptococcus, Blastoschizomyces, and Saccharomyces spp. were used to compare two identification techniques, VITEK 2 and ITS2-fragment length polymorphism analysis (ITS2-FLP), with ID32C as the reference method. ID32C identified 58 isolates correctly. ITS2-FLP with Instagene DNA extraction identified 59 isolates. ITS2-FLP combined with boiling-fr...

The yield of fermentation process, bioethanol, is one the important product obtained from renewable energy sources. Yeasts are commonly used in ethanol production due to their high tolerance, productivity, and ability ferment different kinds simple sugars. Saccharomyces cerevisiae yeast species most industrial production. Therefore, isolation identification new strains with capacity very for bi...

The medicinal plant Ferula has been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics, Ferula is difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use of Ferula. In this study, internal transcribed spacer 2 (ITS...

aroa is an important gene which produces aromatic amino acids essential for bacterial life. the sequence of this gene was shown to be different in bacteria, so the purpose of this study was sequencing of aroa gene in native e. coli o78: k80 to determine the putative differences between this strain and other e. coli o78: k80 strains in gene bank. pcr was used to amplify aroa gene in native e. co...

Primary and secondary structural data from the internal transcribed spacer two (ITS2) have been used extensively for diversity studies of many different eukaryotic organisms, including the green algae. Ease of amplification is due, at least in part, to the fact that ITS2 is part of the tandemly-repeated rRNA array. The potential confounding influence of intragenomic variability has yet to be ad...

The aims of this study were to compare the promoter sequence of the mannose-binding lectin (cMBL) gene in Iranian native and commercial chicken strains; as well as to compare the cMBL gene expression in crossbred and inbred chickens. In total 79 native (Western Azerbaijan native fowls, WANF) and 49 commercial (Arian Commercial Strain, ACS) birds were reared as parents under same management prac...

The population structure of the Chinese carmine spider mite Tetranychus cinnabarinus (Boisduval) was investigated by amplifying and sequencing a portion of the mitochondrial DNA gene coding for cytochrome oxidase I (COI, 453bp) and a portion in the second internal transcribed spacer (ITS2, ̃645bp) of rDNA. Nineteen T. cinnabarinus populations of various geographical origins were sampled from al...

We evaluated a combined panfungal PCR-reverse line blot (RLB) hybridization assay based on internal transcribed spacer 1 (ITS1) and ITS2 region polymorphisms to identify 159 Candida, Cryptococcus neoformans, and Aspergillus isolates (22 species). Its utility to identify fungal pathogens directly from 27 clinical specimens was also determined. ITS sequence analysis was performed to resolve discr...

Abstract Species of Trichogramma Westwood, 1833 (Hymenoptera: Trichogrammtidae) are frequently used as biological control agents against Lepidoptera, but practical application these egg endoparasitoids complicated because their complex taxonomy. This study aimed to compare sequences internal transcribed spacer regions ribosomal DNA (ITS2-rDNA) accessions with those deposited in GenBank order ac...

Astragalus L. is one of the largest angiosperm complex genera that belongs to family Fabaceae, subfamily Papilionoideae or Faboideae under subtribe Astragalinae tribe Galegeae. The current study includes whole plant morphology, DNA barcode (ITS2), and molecular marker (SCoT). Ten taxa representing four species were collected from different localities in Egypt during period February 2018 May 201...