Ticks are important acarina that infest animals. They are obligatory blood sucker arthropods which economically impact cattle industry by reducing weight gain and production. Moreover, they are important vectors of viral, bacterial, rickettsial and parasitic pathogens infecting humans and animals. In view of the importance of Hyalomma anatolicum anatolicum in pathogen transmission, including Th...

background : phlebotomus sergenti s.l. is considered the most likely vector of leishmania tropica in iran. although two morphotypes- p. sergenti sergenti (a) and p. sergenti similis (b)-have been formally described, further morphologi­cal and a molecular analysis of mitochondrial cytochrome oxidase i (mtdna-coi) gene revealed inconsistencies and suggests that the variation between the morphotyp...

OBJECTIVE To identify the etiologic agent from a paragonimiasis patient using molecular techniques. METHODS The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product. ITS2 genes from eggs of Paragonimus westermani and Pagumogonimus skrjabini (both from anim...

ticks are important acarina that infest animals. they are obligatory blood sucker arthropods which economically impact cattle industry by reducing weight gain and production. moreover, they are important vectors of viral, bacterial, rickettsial and parasitic pathogens infecting humans and animals. in view of the importance of hyalomma anatolicum anatolicum in pathogen transmission, including th...

Analysis of sequence variations among isolates of Pneumocystis carinii f. sp. macacae from 14 Indian rhesus monkeys (Macaca mulatta) at the internal transcribed spacer (ITS) regions of the nuclear rRNA gene was undertaken. Like those from P. carinii f. sp. hominis, the ITS sequences from various P. carinii f. sp. macacae isolates were not identical. Two major types of sequences were found. One ...

Background: Traditionally, morphological features of Rhipicephalus (Boophilus) annulatus from closely-related ticks have been considered for their identification and differentiation. However, it is difficult and requires expertise in order to accurately identify and differentiate engorged female ticks and some developmental stages such as larva and nymph from other similar ticks. Hence, molecul...

The ITS2 gene class shows a high sequence divergence among its members that have complicated its annotation and its use for reconstructing phylogenies at a higher taxonomical level (beyond species and genus). Several alignment strategies have been implemented to improve the ITS2 annotation quality and its use for phylogenetic inferences. Although, alignment based methods have been exploited to ...

The Gibberella fujikuroi species complex with Fusarium anamorphs is the causal of diverse diseases of many important plants. Many of the known species in this species complex produce a wide range of secondary metabolites. In this study, morphological characteristics of 46 isolates from rice, maize, sugarcane and onion and phylogenetic relationships of 22 isolates on the basis of ITS1-5.8S-ITS2 ...

30 milled rice samples were collected from retailers in four states of Malaysia. These samples were evaluated for Eurotium spp. contaminations by direct plating on malt extract salt agar (MESA). All Eurotium were isolated and identified based on morphology and nucleotide sequences of internal transcribed spacer 1 (ITS1) and ITS2 of the rDNA.  Four Eurotium species (E. rubrum, E. amstelodami, E....